Abstract: Shoot apices from seeding and adult plants of C.scabrifolia were used. In adult plants, the axillary buds were better than the terminal buds when used us explants to induce clustered shoots. All the buds either from seedings or adult plants can be cultured on MS basal medium supplemented with IAA 0.1- 0.5 mg/l and 6--BA 1 -2 mg/l to induce clustered shoots. MS basal medium with the addition of 0.5-1.0 mg/l 6--BA was used, when subculture. In 40 days, the number of shoots multiples 4 -6 times. Shoots 1.5- 2 cm long can be cut down and cultured in MS/2 basal medium with IAA or IBA 0.05--1 mg/l to induce roots. In 20 days, the percentage of rooting shoots reaches about 80% for shoots derived from adult plants and above 98% from seedlings.

Key words: Crataegus scabrifolia