Plant Diversity ›› 1987, Vol. 9 ›› Issue (03): 1-3.

• Articles •    


Tan Wencheng, Dai Cegang   

  1. Department of Biology, Sichuan Normal University, Chengdu
  • Online:1987-06-25 Published:1987-06-25

Abstract: Abstract This report describes the rapid propagation of Caladium hortulanum and C. bicolor (three cultivars) by using tissue culture. First of all, take the young leaf and petiole as explants and transferred on Murashige and Skoog's agar medium supplemented with benzyladenine 2 mg/l and naphthalene acitc acid 0.6- 1 mg/l, most of them conduced caltus on the cut surface within 2- 3 weeks. Without subculturing 5th weeks it was discovered much globularity and smooth embryoids that differetiated on the surface of callus. They continued to develop and grow heart-shaped leaflet, then rooted, formed complete regenerate plantlets. Results showed that the light and darkness condititions didn't effect the callus conduct and embryogenesis. The second period we suggested by method of static culture in liquid medium (MS+BA 1, omitted agar) which enabled the prolifera- tion speed of the clonal plantlets of Caladium to 3- 4 times per month, and estimate the maltiplication rate acheved about 3.4 x 10^8 times per yeay.

Key words: Caladium