Plant Diversity ›› 1986, Vol. 8 ›› Issue (03): 1-3.
• Articles •
Xu Rujun, Ji Benren and Duan Jinyu
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Abstract: Enzyme-linked immunoassay for the quntitation of zeatinriboside has been studied. Rabbits' antisera produced against bovine serum albumin con- jugates of zeatinriboside have a high affinity and a high specificity for zeatin- riboside. The antisera show a negligible cross reaction to some cytokinins sttch as kinetin cxcept zeatin. This assay method is sensitive and measuring ranges xtend from 0.25 to 50 pmol/assay of zeatinriboside. The reproducibility is also ood. Due to the high specificity of this method, crude extracts may be used for analysis. The method is simple and rapid. And a lot of samples can be analysed in the same time. The quantity of cytokinins levels (calculated as zeatinriboside) in each part of Hyacinthus orientalis L. is 10-60ng/g. F. W..
Key words: Cytokinin
Xu Rujun, Ji Benren and Duan Jinyu. ENZYME-LINKED IMMUNOASSAY (ELISA) FOR ZEATINRIBOSIDE[J]. Plant Diversity, 1986, 8(03): 1-3.
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URL: https://journal.kib.ac.cn/EN/
https://journal.kib.ac.cn/EN/Y1986/V8/I03/1