Plant Diversity ›› 1986, Vol. 8 ›› Issue (03): 1-3.

• Articles •    


Xu Rujun, Ji Benren and Duan Jinyu   

  1. Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204
  • Online:1986-06-25 Published:1986-06-25

Abstract: Enzyme-linked immunoassay for the quntitation of zeatinriboside has been studied. Rabbits' antisera produced against bovine serum albumin con- jugates of zeatinriboside have a high affinity and a high specificity for zeatin- riboside. The antisera show a negligible cross reaction to some cytokinins sttch as kinetin cxcept zeatin. This assay method is sensitive and measuring ranges xtend from 0.25 to 50 pmol/assay of zeatinriboside. The reproducibility is also ood. Due to the high specificity of this method, crude extracts may be used for analysis. The method is simple and rapid. And a lot of samples can be analysed in the same time. The quantity of cytokinins levels (calculated as zeatinriboside) in each part of Hyacinthus orientalis L. is 10-60ng/g. F. W..

Key words: Cytokinin