Abstract: Four new steroidal glycosides named wattosides B-E(1-4) were isolated from the rhizomes of Tupistra wattii. Three of them possess the furostanol skeleton bearing multi-hydroxyl functions. Another one is a cardiac glycoside. The structures of new glycosides have been elucidated by spectroscopic and chemical methods as 22-O-methyl-25(R,S)-furost-1β,3β,4β,5β,22ξ,26β-hexol-26-O-β-D-glucopyranoside(1);22-O-methyl-26-O-β-D-lucopyranoside-25(S)-furost-5-en-1β,3β,22ξ,26β-tetrol-3-O-[O-β-D-lucopyranoside(1-4)]-β-D-galactopyranoside(2);22-O-methyl-25(S)-furost-1β,2β,3β,4β,5β,22,26β-heptol-26-O-β-D-glucopyranoside(3)and oleandrigenin-3-O-[O-β-D-葡萄吡喃糖基(1-2)]-α-L-glucopyranosyl.

Key words: Tupistra wattii