Abstract: Two new C27 steroidal glycosides nominated cixi-ophiopogon A (2) and B(3) together with a known ophiogenin 3-O-α-L-rhamnopyranosyl(1→2)-β-D-glucopyranoside(1)were isolated from the tubers of famous traditional Chinese herb-Ophiopogon japonicum.The spectroscopic and chemical evidences revealed their structures to be ophiogenin 3-O-α-L-rhamnopyranosyl(1→2)［β-D-xylopyranosyl(1→3)］-β-D-glucopyranoside(2) and ophiogenin 3-O-α-L-rhamnopyranosyl(1→2)［β-D-xylopyranosyl(1→3)][β-D-glucopyranosyl(1→4)］-β-D-respectively(3).

Key words: Liliaceae, Ophiopogon japonicum, C27 steroidal glycosides, Cixi-ophiopogon A and B