Abstract: After pollinating for 15 weeks, Cypripedium flavum seeds were collected and bleached in 0.5% NaClO solution for 10 minutes, then sowed in media axenically. After 20 weeks, the seed germination rate reached 90%. Medium, exogenous cytokinin and bleaching is vital to the germination. The mechanism of KT and BA improving germination rate is to act as a direct germination inducer, in addition to be an inhibitor of ABA. Seed coat is one of the important factors in the gevmination of Cypripedium flavum seeds.

Key words: Cypripedium flavum