Plant Diversity ›› 2010, Vol. 32 ›› Issue (4): 347-354.DOI: 10.3724/SP.J.1143.2010.10038

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Profiling the Changes of Molecular Species in Membrane Lipids during Coldacclimation in Winter and Spring Cultivars of Rapeseed (Brassica napus)

 LI  Li-Xia-1,2, LI  Wei-Ai-1   

  1. 1 Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China;
    2 Graduate University of Chinese Academy of Sciences, Beijing 100049,China
  • Received:2010-02-24 Online:2010-08-25 Published:2010-04-07


The changes of molecular species in membrane lipids during coldacclimation (CA) in winter and spring cultivars of rapeseed (Brassica napus) were profiled with ESIMS/MS based lipidomics. The membrane fluidity, the critical properties for plants to tolerate freeing injuring, was examined with double bond index (DBI) and carbon number of the fatty acid of the glycrolipid. The results indicated that the molecular composition of membrane lipids in the two cultivars under both normal growth condition and CA were similar to that of Arabidopsis reported previously and that DBI in spring cultivar accumulation subtly more than that in winter cultivar. The results suggested that CA of rapeseeds needs more than 3 days and that spring cultivar were more sensitive to CA, which was confirmed by the CAinduced proline accumulation.

Key words:  Brassica napus, Coldacclimation, Freezing tolerance, Membrane lipids, Lipid molecular species, Double band index

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