Phylogeographical Study on Primula poissonii (Primulaceae) from Hengduan Mountains

doi:10.3724/SP.J.1143.2011.10247

Abstract

Abstract:

The topological effect of the Hengduan Mountains (HM) during the Quaternary climatic oscillation must have important effects on spatial distribution and genetic structure of plants distributed there. In this study, we examined the chloroplast trnL-F and trnT-L sequence variation of Primula poissonii, a relatively common alpine perennial endemic to this region. Eleven haplotypes were detected by analyzing 167 individuals from 13 populations of this species. Only three of them are commonly shared while the others are respectively fixed in the single population. An analysis of molecular variance (AMOVA) for populations of P.poissonii showed that the genetic variation mainly resided among populations (99.08%), and the estimates of interpopulation differentiation were very high (GST =0.916, FST=0.99077, NST=0.973). Further, a highly significant phylogeographic structure was present (NST>GST, P<0.01) whereas the value of average gene flow was low (Nm=0.08) in the entire geographical range. Along with the nested clade analysis (NCA), our results indicate there were multiple refugia for this species during the glacial stages. We failed to detect the interglacial or postglacial range expansion in this species as commonly revealed for the other temperate plants. The nested clade analysis indicated that allopatric fragmentation was likely the major processes that shaped the present spatial distribution of haplotypes in this species. The special phylogeographic structure of P.poissonii may have resulted from a combination of both climatic oscillation and complex topology of HM.

Key words: Hengduan Mountains, Primula poissonii, Phylogeography, Chloroplast DNA, trnL-F, trnT-L

CLC Number:

Q 16
Q 948

SONG Min-Shu-, LE Ji-Pei-, SUN Hang-, LI Zhi-Min. Phylogeographical Study on Primula poissonii (Primulaceae) from Hengduan Mountains[J]. Plant Diversity, 2011, 33(01): 91-100.

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