Plant Diversity ›› 2013, Vol. 35 ›› Issue (2): 170-172.DOI: 10.7677/ynzwyj201312093

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Efficient Induction of Callus and Plant Regeneration from Miscanthus sacchariflorus Young Ear

 WANG  Qing-Yun, CHENG  Hong-Yan, WANG  Wei-Qing, SONG  Song-Quan   

  1. Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
  • Received:2012-06-21 Online:2013-03-25 Published:2012-08-21
  • Supported by:

    国家科技支撑计划 (2012BAC01B05)


 Plants belonging to the genus Miscanthus are considered promising bioenergy crops. Young ears of Miscanthus sacchariflorus (Maxim.) Benth. were used as explants, and its regeneration system was successfully established via callus pathway. The results showed that Murashige and Skoog (MS) medium containing 600mg·L-1 proline, 500mg·L-1 hydrolyzed casein, 2.0mg·L-1 2, 4D and 0.1mg·L-1 6BA was an optimal medium for induction and growth of callus from young ears. The shoots were induced on MS medium containing 1.5mg·L-1 6BA via callus. The roots were induced after shoots were transferred to 1/2 MS medium containing 0.25mg·L-1 NAA and 0.25mg·L-1 IAA, after that, the young plants were cultivated and grew well on the nutrient soil.

Key words:  Callus pathway, Miscanthus sacchariflorus, Regeneration system, Young ear

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