Plant Diversity ›› 2013, Vol. 35 ›› Issue (3): 348-354.DOI: 10.7677/ynzwyj201313037

• Articles • Previous Articles     Next Articles

Intraspecific Variation of Leaf Epidermal Cuticle Waxes under Scanning Electronic Microscope:Stipa purpurea and Oxytropis microphylla from the QinghaiTibet Plateau

WANG  Dong-Chao-1、2, WU  Jie-3、5, YANG  Yong-Hong-1, CHEN  Jia-Hui-2、3、4, YANG  Yong-Ping-3、4   

  1. 1 Key Laboratory for Plant Pathology of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China; 2 Key
    Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming  650201, China;
    3 Plant Germplasm and Genomics Center, China Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy
    of Sciences, Kunming 650201, China; 4 Institute of Tibetan Plateau Research at Kunming, Chinese Academy of Sciences,
    Kunming  650201, China; 5 University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2013-03-15 Online:2013-05-25 Published:2013-04-15
  • Supported by:

    科技部科技基础性工作专项重点项目 (2007FY110100);国家自然科学基金 (30900085);云南省自然科学基金 (2010CD109);国家重点基础研究发展计划 (2010CB951704)


Leaf epidermal microfeatures under Scanning Electronic Microscope (SEM), especially microfeatures of cuticle waxes, are considered to be a valuable character in plant taxonomy and systematics. Previously studies of leaf epidermal microfeatures mainly focused on levels of species or above species, whereas intraspecific study was rare. In order to explore intraspecific variation of leaf epidermal microfeatures, we selected multiple materials of Stipa purpurea and Oxytropis microphylla (29 and 15 samples, respectively) to observe the microfeatures of leaf cuticle waxes under SEM. The results revealed that these two species bearing high intraspecific variations of microfeatures of leaf cuticle waxes, and these variations lack of a strong correlation with any single biotic or abiotic factors, in contrast, it is affected by combined effects of multiple factors. Therefore, the use of microfeature of leaf cuticle waxes in taxonomical and systematic study will depend, to great extent, upon the detection of microfeature variation range, by extensive sampling along the whole distribution area.

Key words: Qinghai-Tibet Plateau, Leaf epidermis, Cuticle waxes, Intraspecific variation, Stipa purpurea, Oxytropis microphylla

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