AFLP Fingerprinting of Elite Varieties ( clones) from the Genus Populus ( Populaceae)

Abstract

Abstract:

Accurate identification of varieties ( clones) and understanding their genetic relationships are essential for the poplar breeding and variety management. In this study, 21 elite poplar varieties of Tacamahaca and Aigeiros were fingerprinted using AFLP markers. Four AFLP- primer pairs developed generated totally 181 AFLP polymorphic fragments, and in particular, each primer pair generated fingerprint profiles specific to each of the tested varieties. The genetic relationships among the varieties were evaluated by dendrograms and Multidimensional Scaling (MDS) , showing that the tested poplar can be classified into five groups. The results also indicated the clear- cup separation of varieties of different sections of poplar and the primary distinction between native and exotic poplar varieties. Present study indicated that tested poplar varieties could be identified by their fingerprint profiles and that genetic relationships deducted from the varieties study were consistent with their genealogy. In addition, our results demonstrated that AFLP could be used to construct DNA fingerprints of poplar clones at large- scale level and to determine genetic relationships of poplar varieties.

Key words: Populus , Varieties ( clones) , AFLP, Fingerprint, Genetic relationships

CLC Number:

Q 943

GAO Jian-Ming-, Zhang-Shou-Gong-, Ji-Li-Wang-, Zhang-Yong-, Wang-Chun-Guo-, Chen-Rui-Yang-, Song-Wen-Qin-**. AFLP Fingerprinting of Elite Varieties ( clones) from the Genus Populus ( Populaceae)[J]. Plant Diversity, 2006, 01(01): 85-90.

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