Molecular systematics is widely used to determine organismal relationships. However, until now, systematics of angiosperms has mostly employed organellar genes, but these genes are generally inherited uniparentally, only partially reflecting the evolutionary history. In addition, chloroplast and mitochondrial genes are highly conserved, with limited numbers of phylogenetically informative characters. In contrast, nuclear genes are inherited biparentally and have relatively large numbers of informative sites, but only few nuclear genes have been utilized. In this work, sequences of five lowcopy nuclear genes were obtained in 17 species from Brassicaceae and used for phylogenetic analyses with the MaximumParsimony, MaximumLikelihood and Bayesian methods. Highly similar topologies were obtained using the five genes; in addition, compared with organellar genes, the nuclear genes had more phylogenetically informative sites and provided stronger supports. Specifically, the supporting values of all the nodes were 100% in MP trees using the five nuclear genes.Therefore,these five genes are excellent candidate marker genes for plant taxonomists who aim to resolve the relationships among lowlevel taxonomic hierarchies and might also be useful for DNA barcoding.