Plant Diversity ›› 2011, Vol. 33 ›› Issue (6): 674-682.doi: 10.3724/SP.J.1143.2011.11060

• Articles • Previous Articles     Next Articles

Using DNA Barcoding in Genus Tacca (Dioscoreaceae)

 DIAO  Yue-Mei-1、2, ZHANG  Ling-1   

  1. 1 Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla 666303, China;
    2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2011-04-11 Online:2011-12-25 Published:2011-07-05

Abstract:

Tacca (Dioscoreaceae) is a small genus which is taxonomically difficult because of a lack of obvious morphologically differences among species. DNA barcoding is a new method of rapid species identification and discovery using short, standardized genes or DNA regions. In this study, four candidate DNA barcoding regions that three (rbcL, matK, trnHpsbA) from chloroplast genome and one (ITS) from the nuclear genome, were evaluated among 36 accessions representing 6 species of Tacca. The capability of those regions were evaluated by intra and interspecific divergence and barcode gap analyses, and the identification efficiency was assessed using Treebased and BBA methods. The results indicated that both ITS and the core barcode rbcL+matK proposed by CBOL exhibited the highest resolution as a single and combined data respectively. Based on overall performance: matK+rbcL can be considered as a potential barcode for identifying the species of Tacca, ITS can be used as a supplementary barcode. DNA barcoding revealed two distinct lineages of T.integrifolia distributed allopatrically in Tibet and Malaysia. And these two lineages with morphological variations may potentially represent new species.

Key words: Tacca, DNA barcoding, ITS, matK, rbcL, trnH-psbA

CLC Number: 

  • Q 75,Q 949