Phyllosphere eukaryotic microalgal communities in rainforests: Drivers and diversity

doi:10.1016/j.pld.2022.08.006

Plant Diversity ›› 2023, Vol. 45 ›› Issue (01): 45-53.DOI: 10.1016/j.pld.2022.08.006

Phyllosphere eukaryotic microalgal communities in rainforests: Drivers and diversity

Ben-Wen Liu^a, Shu-Yin Li^b, Huan Zhu^a, Guo-Xiang Liu^a

a. Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;
b. Yangtze River Basin Ecological Environment Monitoring and Scientific Research Center, Yangtze River Basin Ecological Environment Supervision and Administration Bureau, Ministry of Ecological Environment, Wuhan 430072, China

收稿日期:2022-03-31 修回日期:2022-08-26 发布日期:2023-02-23
通讯作者: Guo-Xiang Liu,E-mail:liugx@ihb.ac.cn
基金资助:
This work was supported by the National Natural Science Foundation of China (Grant no. 31870189 and 32000168).

Phyllosphere eukaryotic microalgal communities in rainforests: Drivers and diversity

Ben-Wen Liu^a, Shu-Yin Li^b, Huan Zhu^a, Guo-Xiang Liu^a

a. Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;
b. Yangtze River Basin Ecological Environment Monitoring and Scientific Research Center, Yangtze River Basin Ecological Environment Supervision and Administration Bureau, Ministry of Ecological Environment, Wuhan 430072, China

Received:2022-03-31 Revised:2022-08-26 Published:2023-02-23
Contact: Guo-Xiang Liu,E-mail:liugx@ihb.ac.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (Grant no. 31870189 and 32000168).

摘要/Abstract

摘要： Phyllosphere algae are common in tropical rainforests, forming visible biofilms or spots on plant leaf surfaces. However, knowledge of phyllosphere algal diversity and the environmental factors that drive that diversity is limited. The aim of this study is to identify the environmental factors that drive phyllosphere algal community composition and diversity in rainforests. For this purpose, we used single molecule real-time sequencing of full-length 18S rDNA to characterize the composition of phyllosphere microalgal communities growing on four host tree species (Ficus tikoua, Caryota mitis, Arenga pinnata, and Musa acuminata) common to three types of forest over four months at the Xishuangbanna Tropical Botanical Garden, Yunnan Province, China. Environmental 18S rDNA sequences revealed that the green algae orders Watanabeales and Trentepohliales were dominant in almost all algal communities and that phyllosphere algal species richness and biomass were lower in planted forest than in primeval and reserve rainforest. In addition, algal community composition differed significantly between planted forest and primeval rainforest. We also found that algal communities were affected by soluble reactive phosphorous, total nitrogen, and ammonium contents. Our findings indicate that algal community structure is significantly related to forest type and host tree species. Furthermore, this study is the first to identify environmental factors that affect phyllosphere algal communities, significantly contributing to future taxonomic research, especially for the green algae orders Watanabeales and Trentepohliales. This research also serves as an important reference for molecular diversity analysis of algae in other specific habitats, such as epiphytic algae and soil algae.

关键词: Full-length 18S rDNA sequences, Cryptic diversity, Environmental factors, High-throughput sequence, Phyllosphere algae, Tropical rainforest

Abstract: Phyllosphere algae are common in tropical rainforests, forming visible biofilms or spots on plant leaf surfaces. However, knowledge of phyllosphere algal diversity and the environmental factors that drive that diversity is limited. The aim of this study is to identify the environmental factors that drive phyllosphere algal community composition and diversity in rainforests. For this purpose, we used single molecule real-time sequencing of full-length 18S rDNA to characterize the composition of phyllosphere microalgal communities growing on four host tree species (Ficus tikoua, Caryota mitis, Arenga pinnata, and Musa acuminata) common to three types of forest over four months at the Xishuangbanna Tropical Botanical Garden, Yunnan Province, China. Environmental 18S rDNA sequences revealed that the green algae orders Watanabeales and Trentepohliales were dominant in almost all algal communities and that phyllosphere algal species richness and biomass were lower in planted forest than in primeval and reserve rainforest. In addition, algal community composition differed significantly between planted forest and primeval rainforest. We also found that algal communities were affected by soluble reactive phosphorous, total nitrogen, and ammonium contents. Our findings indicate that algal community structure is significantly related to forest type and host tree species. Furthermore, this study is the first to identify environmental factors that affect phyllosphere algal communities, significantly contributing to future taxonomic research, especially for the green algae orders Watanabeales and Trentepohliales. This research also serves as an important reference for molecular diversity analysis of algae in other specific habitats, such as epiphytic algae and soil algae.

Key words: Full-length 18S rDNA sequences, Cryptic diversity, Environmental factors, High-throughput sequence, Phyllosphere algae, Tropical rainforest

Ben-Wen Liu, Shu-Yin Li, Huan Zhu, Guo-Xiang Liu. Phyllosphere eukaryotic microalgal communities in rainforests: Drivers and diversity[J]. Plant Diversity, 2023, 45(01): 45-53.

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Phyllosphere eukaryotic microalgal communities in rainforests: Drivers and diversity

Phyllosphere eukaryotic microalgal communities in rainforests: Drivers and diversity

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