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Plant Diversity ›› 2009, Vol. 31 ›› Issue (1): 67-74.DOI: 10.3724 SP.J.1143.2009.08127

• 研究论文 • 上一篇    下一篇

饱和白光引起的光系统II 捕光复合体(LHCII ) 从反应中心复合体脱离不同于弱红光引起的状态1 向状态2 的转换

陈 悦1 , 2 , 许大全1   

  1. 1 中国科学院上海生命科学研究院植物生理生态研究所, 上海 200032;
    2 中国科学院研究生院, 北京 100049
  • 收稿日期:2008-06-23 出版日期:2009-02-25 发布日期:2009-02-25
  • 通讯作者: 许大全

Dissociation of Photosystem II Light-harvesting Complex (LHC II ) from the Reaction Center Complex Induced by Saturating White Irradiation Differs from the Transition from State1 to State 2 Induced by Weak Red Irradiation

CHEN Yue1 , 2 , XU Da-Quan1   

  1. 1 Institute of Plant Physiology and Ecology , Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032 , China ; 2 Graduate University of Chinese Academy of Sciences, Beijing 100049 , China
  • Received:2008-06-23 Online:2009-02-25 Published:2009-02-25
  • Contact: XU Da-Quan

摘要: 我们观测了不同光照预处理对拟南芥、小麦和大豆叶片光合作用和低温( 77K) 叶绿素荧光参数F685、F735 和F685􊄯F735 的影响。野生型拟南芥叶片光合作用对饱和光到有限光转变的响应曲线是V 型,而缺乏叶绿体蛋白激酶的突变体STN7 的这一曲线为L 型。饱和白光可以引起拟南芥叶片F685􊄯F735 的明显降低, 但是F735 没有明显增高, 而弱红光可以导致拟南芥叶片F685􊄯F735 的明显降低和F735 的明显增高, 表明弱红光可以引起状态1 向状态2 的转变, 同时伴随从光系统II 脱离的LHC II 与光系统I 的结合, 而饱和白光只能引起LHC II 从光系统II 反应中心复合体脱离。并且, 低温叶绿素荧光分析结果证明, 饱和白光可以引起大豆叶片LHC II 脱离, 但是不能引起小麦叶片LHC II 脱离, 而弱红光可以引起小麦叶片的这种状态转换, 却不能引起大豆叶片的这种状态转换。因此, 饱和白光引起的野生型拟南芥和大豆叶片的LHC II 脱离不是一个典型的状态转换现象。

关键词: 拟南芥, 光系统II 捕光复合体(LHC II) , 低温(77K) 叶绿素荧光, 净光合速率, 可逆脱离,

Abstract: The effects of different irradiance pre-treatments on leaf photosynthesis and low temperature ( 77K) chlorophyll
fluorescence (LTCF) parameters F685 , F735 , and F685􊄯F735 were observed in Arabidopsis, wheat , and soybean leaves
. The curve of photosynthetic responsing to irradiance transition from saturating to limiting one in Arabidopsis wild- type
leaves was the V pattern , while the curve in Arabidopsis mutant lacking chloroplast protein kinase STN7 was the L pattern .
Saturating white irradiation (SWI ) could induce the significantly decreased F685􊄯F735 without a significantly increased
F735 , while weak red irradiation (WRI) could lead to the significantly declined F685􊄯F735 with a significantly increased
F735 . The results showed that the attachment of dissociated LHC II to PS I in Arabidopsis wild-type leaves , indicating that
WRI can cause the transition from state 1 to sate 2 , while SWI can only induce LHC II dissociation from PSII reaction cen-ter complex . Moreover the results of LTCF analysis demonstrated that SWI could induce the LHC II dissociation in soybean
but not wheat leaves , while WRI could cause the state transition in wheat but not soybean leaves . Hence the LHC II dissociation
caused by SWI in Arabidopsis wild-type and soybean leaves was not a typical phenomenon of the state transition .

Key words: Arabidopsis