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Plant Diversity ›› 2010, Vol. 32 ›› Issue (01): 25-31.DOI: 10.3724/SP.J.1143.2010.09140

• 研究论文 • 上一篇    下一篇


王卫清1, 2,成晓1, 焦瑜1,陈贵菊3


  1. 1中国科学院昆明植物研究所, 云南 昆明650204; 2 中国科学院研究生院, 北京100049;
    3 济宁市农业科学研究院, 山东 济宁272031
  • 收稿日期:2009-07-28 出版日期:2010-02-25 发布日期:2010-02-25
  • 通讯作者: 成晓

Substratum Affects the Gametophyte Development and Reproduction of Sinopteris grevilleoides(Sinopteridaceae), an Endangered Fern Endemic to China

WANG WeiQing 1, 2,CHENG Xiao1*, JIAO Yu1 ,CHEN GuiJu3   

  1. 1Botanical Garden, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China; 2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China;3 Jining Institute of Agricultural Sciences, Jining 272031, China
  • Received:2009-07-28 Online:2010-02-25 Published:2010-02-25
  • Contact: CHENG Xiao

摘要: 采用4种培养基质对中国蕨(Sinopteris grevilleoides)孢子进行培养,结果显示:不同基质上中国蕨配子体和幼孢子体生长发育存在差异,尤其是心形原叶体的形态差别明显;腐叶土基质上的心形原叶体上未见颈卵器的发生,因而其有性繁殖过程不能完成;原生境土和腐叶土1∶1混合土基质最适合中国蕨配子体生长发育及有性繁殖;改良Knop′s琼脂培养基上要保证幼孢子体正常生长需追加培养液。

关键词: 中国蕨; 配子体;形态发育; 繁殖;培养基质

Abstract: Sinopteris grevilleoides is an endangered fern endemic to the region of Yunnan and Sichuan in southwest China. In this study, we investigated the effects of four different culture substrata on the development of gametophyte and reproduction of this fern. Cultured on different culture substrata, the fern showed different morphology of gametophyte. In humus soil, cordate prothalli did not bear archegonia, and thus the process of sexual reproduction ofSgrevilleoides could not be finished. The substratum composed of humus soil and original soil in ratio of 1∶1 was the most suitable substratum for gametophyte development and sexual reproduction of this species. Otherwise, infant sporophytes needed extra nutrient solution to sustain its growth on improved Knop′s agar medium.

Key words: Sinopteris grevilleoides