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Plant Diversity ›› 2013, Vol. 35 ›› Issue (6): 693-700.doi: 10.7677/ynzwyj201313186

• 研究论文 • 上一篇    下一篇

应用DNA条形码对植物标本的核查

曾春霞,贺正山,杨俊波   

  1. 中国科学院昆明植物研究所中国西南野生生物种质资源库,云南 昆明650201
  • 收稿日期:2013-09-11 出版日期:2013-11-25 发布日期:2013-10-16
  • 基金资助:

    科技部科技基础性工作专项项目 (2013FY112600);国家高技术研究发展计划 (863计划) 主题项目 (2012AA021801);中国科学院仪器设备功能开发技术创新项目实施方案项目 (植物遗传信息高效获取技术体系集成研制)

DNA Barcoding for Plant Specimen Identification*

 ZENG  Chun-Xia, HE  Zheng-Shan, YANG  Jun-Bei   

  1. Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
  • Received:2013-09-11 Online:2013-11-25 Published:2013-10-16

摘要:

DNA条形码主要目的是物种鉴定和新物种或隐存种的发现,而DNA条形码参考数据库是物种快速鉴定的重要基础。目前中国维管植物DNA条形码参考数据库正在建设之中,借助于公共数据库(NCBI)和初步建立的中国植物DNA条形码参考数据库,运用DNA条形码数据开展了植物标本鉴定的核查工作:(1) 比较DNA序列信息与标本鉴定信息,从科、属、种级水平查找鉴定错误的标本;(2) 基于有较好研究基础的DNA条形码参考数据库,开展未知标本的鉴定;(3) 通过对标本核查的总结,提出DNA条形码参考数据库建设过程中的几点建议。

关键词: 植物, 标本鉴定, DNA条形码, 参考数据库

Abstract:

 DNA barcoding comprises two distinct aims: specimen identification and species discovery. To achieve these goals, a DNA barcode reference library is indispensable and is currently under construction for Chinese plants. In this paper, we used the existing public database, NCBI, and also our DNA barcode reference library, which is under construction, to identify plant specimens. By combining DNA barcode sequences with scientific names determined by taxonomists, we first attempted to find incorrectly identified specimens and also to check specimens whose DNA sequences were wrongly labeled. Secondly, we attempted the identification of unknown specimens in some groups for which DNA barcodes are available. Thirdly, we make some suggestions for the development of a plant DNA barcode reference library.

Key words: Plants, Specimen identification, DNA barcoding, Reference library

中图分类号: 

  • Q 949