[an error occurred while processing this directive]

Plant Diversity ›› 2015, Vol. 37 ›› Issue (01): 1-10.DOI: 10.7677/ynzwyj201514052

• 研究论文 •    下一篇

本地种云南柳与外来种旱柳 (杨柳科) 的同倍体自然杂交

 吴杰1、2、3、4, 王东超1、2、3, 杨永平1、2、3, 陈家辉1、2、3   

  1. 1 中国科学院昆明植物研究所东亚植物多样性与生物地理学重点实验室,昆明650201;2 中国科学院
    昆明植物研究所西南野生生物种质资源库,昆明650201;3 中国科学院昆明植物研究所
    青藏高原研究所昆明部,昆明650201;4 中国科学院大学,北京100049
  • 收稿日期:2014-03-27 出版日期:2015-01-25 发布日期:2014-06-10
  • 基金资助:

    The National Natural Science Foundation of China (NSFC 31270271 to JH. Chen), the Project of Knowledge Innovation Program of the Chinese Academy of Sciences (Grant No.: KSCX2EWJ24), Yunnan Natural Science Foundation (2010CD109 to JH. Chen), and the Youth Innovation Promotion Association, CAS

Homoploid Hybridization between Native Salix cavaleriei and Exotic Salix matsudana (Salicaceae)

 WU  Jie-1、2、3、4, WANG  Dong-Chao-1、2、3, YANG  Yong-Ping-1、2、3, CHEN  Jia-Hui-1、2、3   

  1. 1 Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences,
    Kunming 650201, China; 2 Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany,
    Chinese Academy of Sciences, Kunming 650201, China; 3 Institute of Tibetan Plateau Research at Kunming,
    Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
    4 University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2014-03-27 Online:2015-01-25 Published:2014-06-10
  • Supported by:

    The National Natural Science Foundation of China (NSFC 31270271 to JH. Chen), the Project of Knowledge Innovation Program of the Chinese Academy of Sciences (Grant No.: KSCX2EWJ24), Yunnan Natural Science Foundation (2010CD109 to JH. Chen), and the Youth Innovation Promotion Association, CAS

摘要:

对分布于云南的旱柳 (Salix matsudana) 和云南柳 (Salix cavaleriei) 之间的一个自然杂交种进行了研究。野外观察表明疑似杂交种异蕊柳 (Salix × heteromera) 形态上介于旱柳和云南柳之间,并得到了基于叶形态特征的主成份分析的印证。核基因ITS序列数据表明这三个种存在ITS序列的种内和个体内的多态性,且疑似杂交种的ITS序列的基因型总是疑似亲本的嵌合体。因此可以判定异蕊柳是旱柳和云南柳的自然杂交后代。流式细胞分析表明这三个种均为四倍体,因而,本杂交事件为同倍体杂交。基于四个叶绿体序列片段的数据表明本自然杂交事件是不对称的,云南柳是异蕊柳的母本。常见外来种旱柳与稀有本地种云南柳的杂交可能导致稀有种云南柳的濒危甚至灭绝。研究表明柳属植物的引种应非常谨慎。

关键词: 柳属, 同倍体杂交, 不对称杂交, 分子证据

Abstract:

Natural hybridization between Salix matsudana and Salix cavaleriei was investigated based on populations from Yunnan, China. Field observations revealed that the putative hybrid, S× heteromera had intermediate morpho
logies between Smatsudana and S. cavaleriei. This was further confirmed by principal component analysis. Sequence data of nuclear rDNA internal transcribed spacer region showed both intraspecific and intragenomic polymorphisms in all the three species, and S× heteromera showed a strong additive pattern between its suspected progenitors at all nucleotide sites of the genotypes identified. Therefore, S× heteromera was confirmed to be a natural hybrid between Scavaleriei and Smatsudana. Flow cytometry analysis indicated that all the three species are tetraploid, and the hybridization was homoploid. Sequence data from four chloroplast datasets indicated that the hybridization was asymmetric, with Scavaleriei as the maternal parent. The hybridization between the exotic common species Smatsudana and native rare species Scavaleriei might increase the risk of endangerment and even extinction, indicating that the introduction of Salix species should be made very cautiously.

Key words:  Salix, Homoploid hybridization Asymmetry, Molecular evidence

中图分类号: