丛枝菌根真菌对百喜草的生理特性的影响

doi:10.3724/SP.J.1143.2011.11007

Plant Diversity ›› 2011, Vol. 33 ›› Issue (5): 521-528.DOI: 10.3724/SP.J.1143.2011.11007

丛枝菌根真菌对百喜草的生理特性的影响

肖家欣1、2, 任群1, 吴雪俊1, 陈迎迎1, 张绍铃2

1 安徽师范大学生命科学学院，生物环境与生态安全安徽省高校省级重点实验室，安徽芜湖241000；
2 南京农业大学园艺学院，江苏南京210095

收稿日期:2011-01-10 出版日期:2011-10-25 发布日期:2011-08-02
基金资助:
Specialized Research Fund for the Doctoral Program of Higher Education(20103424120002), China Postdoctoral Science Foundation (20080430172) and Anhui Provincial Natural Science Foundation (070411004)

Effects of Arbuscular Mycorrhizal Fungi on Physiological Character of Bahia Grass (Paspalum notatum, Poaceae)

XIAO Jia-Xin-1、2, REN Qun-1, Wu Xue-Jun-1, CHEN Ying-Ying-1, ZHANG Shao-Ling-2

1 Key Laboratory of Biotic Environment and Ecological Security, Anhui Province, College of Life Sciences,
Anhui Normal University, Wuhu 241000, China; 2 College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China

Received:2011-01-10 Online:2011-10-25 Published:2011-08-02
Supported by:
Specialized Research Fund for the Doctoral Program of Higher Education(20103424120002), China Postdoctoral Science Foundation (20080430172) and Anhui Provincial Natural Science Foundation (070411004)

摘要/Abstract

摘要：

采用盆栽法研究了丛枝菌根（AM）真菌摩西球囊霉（Glomus mosseae）对水分胁迫条件下百喜草（Paspalum notatum）生长、渗透调节及抗氧化酶的影响。结果表明：接种AM真菌显著提高了百喜草的株高、地上部与根部鲜重、地上部P、K、Mn及根部P、Ca、Mn含量，明显降低了地上部Zn及根部Fe、B、Cu水平；随着干旱程度的加深，接种株的地上部相对含水量及叶绿素含量相对稳定且均显著高于未接种株，接种株地上部相对电导率、MDA含量均显著低于未接种株，接种株的地上部POD活性与脯氨酸含量均显著增加且均显著高于未接种株，AM侵染对SOD活性的影响较小。可见，接种AM真菌Glomus mosseae提高了植株体内保护酶活性（如POD）及渗透调节能力（如脯氨酸、P、K、Ca等渗透调节物含量的增加），从而显著增强了百喜草的抗旱性。

关键词: 丛枝菌根真菌, 百喜草, 抗氧化物质, 渗透调节, 水分胁迫

Abstract:

The effects of arbuscular mycorrhizal (AM) fungus, Glomus mosseae, on growth, osmotic adjustment and antioxidant enzymes of bahia grass (Paspalum notatum) were studied in potted plants under water stress conditions. AM colonization significantly enhanced the plant height, root and shoot fresh weight, Phosphorus (P), potasium (K), manganese (Mn) contents in shoots, P, calcium (Ca), Mn contents in roots, whereas obviously decreased zinc (Zn) content in shoots, iron (Fe), boron (B), copper (Cu) contents in roots. During water stress, the relative water and chlorophyll contents were relatively stable and signifciantly higher in AM than in non-AM plants, AM inoculation notabley decreased the shoot relative conductivity and malondialdehyde (MDA) content, markedly increased shoot peroxidase (POD) activity and proline content, while AM infection did not affect the dismutase (SOD) activity of shoots. Our results suggested that AM colonization improved the protective enzyme activity (such as POD) and osmotic adjustment originating from proline P, K, Ca, resulting in the enhancement of drought tolerance.

Key words: Arbuscular mycorrhizal fungi, Bahia grass, Antioxidant, Osmotic adjustment, Water stress

中图分类号:

Q 945

肖家欣1、2, 任群1, 吴雪俊1, 陈迎迎1, 张绍铃2. 丛枝菌根真菌对百喜草的生理特性的影响[J]. Plant Diversity, 2011, 33(5): 521-528.

XIAO Jia-Xin-, REN Qun-, Wu Xue-Jun-, CHEN Ying-Ying-, ZHANG Shao-Ling. Effects of Arbuscular Mycorrhizal Fungi on Physiological Character of Bahia Grass (Paspalum notatum, Poaceae)[J]. Plant Diversity, 2011, 33(5): 521-528.

参考文献

Auge RM, 2004. Arbuscular mycorrhizae and soil/plant water relations［J］. Canadian Journal of Soil Science, 84: 373—381
Chance B, Maehly AC, 1955. Assay of catalases and peroxidases［J］. Methods Enzymology, 2: 764—775
Fitter AH, 1988. Water relations of red clover Trifolium pratense L. as affected by VA mycorrhizal infection and phosphorus supply before and during drought［J］. Journal of Experimental Botany, 39: 595—603
Giannopolitis CN, Ries SK, 1977. Superoxide dismutase.Ⅰ.Occurrence in higher plants［J］. Plant Physiology, 59: 309—314
Goicoechea N, Antolin MC, Sanchez-Diaz M, 1997. Gas exchange is related to the hormone balance in mycorrhizal or nitrogen-fixing alfafa subjected to drought［J］. Physiologia Plantarum, 100: 989—997
He XL (贺学礼), Liu T (刘媞), An XJ (安秀娟) et al., 2009a. Effects of AM fungi on the growth and drought resistance of Caragana korshinskii under water stress conditions［J］. Acta Ecologica Sinica(生态学报), 29 (1): 47—52
He XL (贺学礼), Liu T (刘媞), Zhao LL (赵丽莉), 2009b. Effects of inoculating AM fungi on physiological characters and nutritional components of Astragalus membranaceus under different N application levels［J］. Chinese Journal of Applied Ecology (应用生态学报), 20 (9): 2118—2122
Kapoor R, Sharma D, Bhatnagar AK, 2008. Arbuscular mycorrhizae in micropropagation systems and their potential applications［J］. Scientia Horticulturae, 116: 227—239
Kramer PJ, Boyer JS, 1997. Water Relations of Plants and Soils［M］. San Diego: Academic Press, 1—20
Lacan D, Baccou JC, 1998. High levels of antioxidant enzymes correlate with delayed senescence in nonnetted muskmelon fruits［J］. Planta, 204: 377—382
Li GH (李国怀), Yin HL (伊华林), Xia RX (夏仁学), 2005. Application of bahia grass(Paspalum notatum) to eco-agriculture construction in Southern China［J］. Chinese Journal of Eco-Agriculture (中国生态农业学报), 13 (4): 197—199
Li GH (李国怀), Yin HL (伊华林), 2005. Influence of sod culture on the soil water content, effect of soil nutrients, fruit yield and quality in citrus orchard［J］. Chinese Journal of Eco-Agriculture (中国生态农业学报), 13 (2): 160—163
Li HK (李会科), Zhang GZ (张广军), Zhao ZY (赵政阳) et al., 2007. Effects of interplanting of herbage on soil nutrient of non-irrigated apple orchard in the loess plateau［J］.Acta Hortculturae Sinica (园艺学报), 34 (2): 477—480
Li HS (李合生), 2003. Experimental principle and technique for plant physiology and biochemistry［M］. Beijing: Higher Edueation Press, 167—169
Miransari M, Bahrami HA, Rejali F et al., 2009a. Effects of soil compaction and arbuscular mycorrhiza on corn (Zea mays L.) nutrient uptake［J］. Soil & Tillage Research, 103: 282—290
Miransari M, Bahrami HA, Rejali F et al., 2009b. Effects of arbuscular mycorrhiza, soil sterilization, and soil compaction on wheat (Triticum aestivum L.) nutrients uptake［J］. Soil ＆ Tillage Research, 104: 48—55
Phillips JM, Hayman DS, 1970. Improve procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection［J］. Transactions of the British Mycological Society, 55: 158—161
Ren Q (任群), Xiao JX (肖家欣), Chen SL (陈世林) et al., 2009. Influences of sod culture on mineral nutrition in citrus leaves and fruit quality［J］.Chinese Agricultural Science Bulletin (中国农学通报), 25 (24): 407—409
Ruiz-Lozano JM, 2003. Arbuscular mycorrhizal symbiosis and alleviation of osmotic stress, new perspectives for molecular studies［J］. Mycorrhiza, 13: 309—317
Ruiz-Lozano JM, Azcon R, 1995. Hyphal contribution to water uptake in mycorrhizal plants as affected by the fungal species and water status［J］. Physiologia Plantarum, 95: 472—478
Song HX (宋会兴), Peng YY (彭远英), Zhong ZC (钟章成), 2008. Photosynthetic responses of AMF infected and AMF-free Bidens pilosa L. to drought stress conditions［J］. Acta Ecologica Sinica (生态学报), 28 (8): 3745—3751
Sudhakar C, Lakshmi A, Giridarakumar S, 2001. Changes in the antioxidant enzymes efficacy in two high yielding genotypes of mulberry (Morus alba L.) under NaCl salinity［J］. Plant Science, 161: 613—619
Troll W, Lindsley JA, 1955. A photometric method for the determination of proline［J］. Journal of Biological Chemistry, 215: 655—660
Wang MY (王明元)，Xia RX (夏仁学), 2009. Effects of arbuscular mycorrhizal fungi on growth and iron uptake of Poncirus trifoliata under different pH［J］. Acta Microbiologica Sinica (微生物学报), 49 (10): 1374—1379
Wu QS (吴强盛), Xia RX (夏仁学), Hu ZJ (胡正嘉), 2005. Effects of arbuscular mycorrhiza on drought tolerance of Poncirus trifoliata［J］. Chinese Journal of Applied Ecology (应用生态学报), 16 (3): 459—463
Wu QS, Xia RX, Zou YN, 2006. Reactive oxygen metabolism in mycorrhizal and non-mycorrihizal citrus (Poncirus trifoliata) seedlings subjected to water stress［J］. Journal of Plant Physiology, 163: 1101—1110
Wu QS, Xia RX, 2006. Arbuscular mycorrhizal fungi influence growth, osmotic adjustment and photosynthesis of citrus under well-watered and water stress conditions［J］. Journal of Plant Physiology, 163: 417—425
Wu QS (吴强盛), Zou YN (邹英宁), 2007. Influence of AM fungi on nutrient uptake in leaves of trifoliate orange seedlings subjected to water stress［J］.Acta Botany Boreal-Occident Sinica (西北植物学报), 27 (1): 0074—0078
Zeng M (曾明), Li DG (李道高), Xiong BQ (熊丙全) et al., 2004. Effects of sod culture management in citrus orchardson AM infection in citrus roots and on fruit quality［J］. Journal of Southwest Agricultural University (西南农业大学学报), 26 (2): 105—107

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丛枝菌根真菌对百喜草的生理特性的影响

Effects of Arbuscular Mycorrhizal Fungi on Physiological Character of Bahia Grass (Paspalum notatum, Poaceae)

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