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Plant Diversity ›› 2011, Vol. 33 ›› Issue (5): 529-534.DOI: 10.3724/SP.J.1143.2011.11064

• 研究论文 • 上一篇    下一篇


 杨春1、2, 刘爱忠1   

  1. 1 中国科学院西双版纳热带植物园,云南 勐腊666303; 2 中国科学院研究生院,北京100049
  • 收稿日期:2011-04-18 出版日期:2011-10-25 发布日期:2011-05-19
  • 基金资助:


Development of EST-SSR Markers from Jatropha curcas (Euphorbiaceae) and Their Application in Genetic Diversity Analysis among Germplasms

 YANG  Chun-1、2, LIU  Ai-Zhong-1   

  1. 1 Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla 666303, China;
    2  Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2011-04-18 Online:2011-10-25 Published:2011-05-19
  • Supported by:



小桐子(Jatropha curcas) 适应性强,不择土壤,种子油脂性能适宜生物柴油的生产,是重要的生物柴油植物。基于小桐子种子发育过程中的EST序列,采用生物信息学方法,从4640个EST非冗余序列上鉴别了1009个SSR位点并分析其分布特征;开发了11对多态的EST-SSR分子标记,并利用这些分子标记调查了24个不同地理种源的遗传多样性,从每个位点的等位基因数目(2~3,平均为2.45)、期望杂合度(He为0.0887~0.5128,平均是0.2736)、多态信息含量(PIC为0.0847~0.4031,平均是0.2313)等方面反映了小桐子种质的遗传多样性低。进一步分析显示不同地理种源的遗传关系缺乏明显的地理结构。作者开发的EST-SSR分子标记不仅有助于小桐子种质的遗传多样性研究,也有助于小桐子种质间的遗传关系鉴别。

关键词: 小桐子, 生物柴油植物, EST-SSR, 遗传多样性


Jatropha curcas (Euphorbiaceae) has created tremendous interest all over the world for the use of its seed oil as a commercial source of biodiesel. Based on 9843 ESTs available from the developing seeds of Jatropha curcas, we identified 1009 SSRs in 4640 unigenes and developed 11 polymorphic EST-SSR markers which exhibited a low level of genetic diversity among germplasms, i.e. allele number varied from 2 to 3, with a average of 2.45; Heterozygosity (He) ranged from 0.0887-0.5128, with a average of 0.2736; Polymorphic Information Content (PIC) ranged from 0.0847-0.4031, with a average of 0.2313. Further, we analyzed the genetic relationships among 24 germplasms collected from different areas in southern China, northern Vietnam, and India using the 11 EST-SSR markers. The results showed that there was no a geographic pattern of genotypes across the collection areas of Jatropha curcas. The EST-SSR markers developed in current study is useful for both genetic diversity analysis and identification of genetic relationships among germplasms in Jatropha curcas.

Key words: Biodiesel plant, EST-SSR, Genetic diversity, Jatropha curcas