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Plant Diversity ›› 2011, Vol. 33 ›› Issue (6): 653-659.DOI: 10.3724/SP.J.1143.2011.11046

• 研究论文 • 上一篇    下一篇

WRKY25过量表达导致拟南芥在长光照下开花提前

 王芳秀1、2, 黎舒佳1、3, 余迪求1   

  1. 1 中国科学院西双版纳热带植物园,云南 昆明650223;2 中国科学院研究生院,北京100049;
    3 中国科学院遗传与发育生物学研究所,北京100101
  • 出版日期:2011-12-25 发布日期:2012-02-24

Overexpression of WRKY25 Causes Early Flowering in Arabidopsis under Longday Conditions

 WANG  Fang-Xiu-1、2, LI  Shu-Jia-1、3, TU  Di-Qiu-1   

  1. 1 Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China;
    2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China; 3 Institute of Genetics
    and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
  • Online:2011-12-25 Published:2012-02-24

摘要:

WRKY基因家族是主要存在于植物中的一大类转录调控因子,拥有很多家族成员。拟南芥WRKY25属于第I类WRKY蛋白,参与植物生物和非生物胁迫反应。通过GUS染色和qRTPCR发现WRKY25基因主要在根,叶和茎生叶中表达。过量表达WRKY25的转基因植株在长光照下比野生型拟南芥提前开花。通过RTPCR检测与开花时间相关基因发现,AP1基因的表达量在培养21d和27d的WRKY25过量表达植株中上调,由此推测WRKY25很可能通过增强AP1的表达来影响开花。

关键词: WRKY25, 过量表达, 长光照, AP1, 提前开花

Abstract:

The WRKY gene superfamily is one of the largest transcriptional regulators which mainly exists in plants. Arabidopsis WRKY25 is one of the group I WRKY proteins and responds to both biotic and abiotic stresses. GUS staining and qRTPCR analysis show that the expression of WRKY25 mainly focuses on roots, rosette leaves and cauline leaves. Transgenic plants overexpressing WRKY25 show early flowering under longday conditions when compared with wildtype plants. RTPCR analysis demonstrates that the expression of AP1 is upregulated in 21dold and 27dold transgenic plants. Thus, it is possible that WRKY25 affects flowering through upregulation of AP1 directly or indirectly.

Key words: WRKY25, Over-expressing, Long-day conditions, AP1, Early flowering

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