竹亚科lea3基因的进化分析

doi:10.7677/ynzwyj201414038

Plant Diversity ›› 2014, Vol. 36 ›› Issue (06): 707-714.DOI: 10.7677/ynzwyj201414038

竹亚科lea3基因的进化分析

于丽霞, 李斌, 黄海泉, 鄢波

西南林业大学园林学院，云南昆明650224

收稿日期:2014-03-13 出版日期:2014-11-25 发布日期:2014-06-13
基金资助:
国家自然科学基金项目 (31160177, 31460202)，云南省部级重点学科、省高校重点实验室及校实验室共享平台资助

The Evolutionary Analysis of lea3 Gene in Bambusoideae

YU Li-Xia, LI Bin, HUANG Hai-Quan, YAN Bo

Southwest Forestry University, Faculty of Landscape Architecture, Kunming 650224, China

Received:2014-03-13 Online:2014-11-25 Published:2014-06-13
Supported by:
国家自然科学基金项目 (31160177, 31460202)，云南省部级重点学科、省高校重点实验室及校实验室共享平台资助

摘要/Abstract

摘要：

选取竹亚科中两个超族、六个族和三个亚族的10个竹种为材料，分别是泰竹、凤尾竹、青皮竹、大叶慈、慈竹、野龙竹、毛竹、香竹、苦竹、菲白竹，分离克隆了它们的lea3基因，并将它们与外类群物种水稻进行序列比对和进化分析。结果发现在分支模型与分支位点模型的检测中，不同竹种所含lea3基因承受了不同的正选择压力，清除选择作用在lea3基因编码区中占主导地位（ω<1）。在位点模型的检测中，共检测出了18个显著性正选择位点，占总氨基酸数目的111%。对这18个显著性正选择位点进行定位后，发现其中的15个位于11个氨基酸串联重复序列附近。这说明lea3基因中的11个氨基酸串联重复序列区比基因其它区域更容易受自然选择作用影响。同时，在位点模型检测结果的基础上，通过对强烈清除选择位点的定位，发现在11个氨基酸串联重复序列区内存在一长段无强烈清除位点的序列区。

关键词: 竹亚科, lea3基因, 基因克隆, 进化分析

Abstract:

The 10 bamboo species for cloning lea3 gene concern two superfamily, six families and three subtribes，they are Thyrsostachys siamensis,Bambusa multiplex, Bambusa textilis, Dendrocalamus farinosus, Neosinocalamus affinis, Dendrocalamus semiscandens, Phyllostachys heterocycla, Chimonocalamus delicates, Pleioblastus amarus, Sasa fortunei. In the branch model and branch site model test, found that different bamboo species containing lea3 gene under different selection pressure, purifying selection function is in dominant (omega<1). In the site model test, detected a total of 18 significant positive selection sites, account for 111% of the total amino acid number. Locating the 18 significant positive selection sites, found 15 sites located 11 amino acid tandem repeat sequences areas, this means that 11 amino acid tandem repeat sequences areas of lea3 gene than in other regions more easily affected by natural selection effect. At the same time, based on the results of the site model test, through located these intence purifying selected sites, found a long no purifying selection sites area in 11 amino acid tandem repeat sequences.

Key words: Bambusoideae, lea3 gene, Gene cloning, Evolutionary analysis

中图分类号:

Q 75

于丽霞, 李斌, 黄海泉, 鄢波. 竹亚科lea3基因的进化分析[J]. Plant Diversity, 2014, 36(06): 707-714.

YU Li-Xia, LI Bin, HUANG Hai-Quan, YAN Bo. The Evolutionary Analysis of lea3 Gene in Bambusoideae[J]. Plant Diversity, 2014, 36(06): 707-714.

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竹亚科lea3基因的进化分析

The Evolutionary Analysis of lea3 Gene in Bambusoideae

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