利用流式细胞仪测定荚蒾属植物的基因组大小

doi:10.7677/ynzwyj201414022

Plant Diversity ›› 2014, Vol. 36 ›› Issue (06): 730-736.DOI: 10.7677/ynzwyj201414022

利用流式细胞仪测定荚蒾属植物的基因组大小

张宁宁1、2, 杨静1, 孙卫邦1

1 中国科学院昆明植物研究所昆明植物园，云南昆明650201；2 中国科学院大学，北京100049

收稿日期:2014-02-07 出版日期:2014-11-25 发布日期:2014-04-24
基金资助:
中国科学院知识创新工程重要方向项目“西南地区重要植物类群分类、评价与挖掘” (KSCX2EWJ24)

Genome Size Estimation of Viburnum (Adoxaceae) Species by Using Flow Cytometry*

ZHANG Ning-Ning-1、2, YANG Jing-1, SUN Wei-Bang-1

1 Kunming Botanical Garden, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; 2 University of Chinese Academy of Sciences, Beijing 100049, China

Received:2014-02-07 Online:2014-11-25 Published:2014-04-24
Supported by:
中国科学院知识创新工程重要方向项目“西南地区重要植物类群分类、评价与挖掘” (KSCX2EWJ24)

摘要/Abstract

摘要：

荚蒾属 (Viburnum) 植物在园林中广泛用作观赏灌木，并且其具有优良园艺性状的杂交种在世界范围内越来越受欢迎。本研究利用流式细胞仪测定了14种荚蒾属植物的基因组大小。二倍体中基因组大小变化范围是255pg（陕西荚蒾）到426pg（琼花）。同时，琼花的核型也较不对称，这可能反应了它的育种历史。四倍体物种珊瑚树的基因组大小（762pg）是其他二倍体物种的两倍还多，这揭示了该属的多倍化在进化中可能并不遥远。该研究为荚蒾属细胞遗传学和分类学的深入研究奠定了基础，并为该属杂交育种提供有用的信息。

关键词: 荚蒾属, 流式细胞仪, 基因组大小, 变异, 染色体

Abstract:

Viburnum species are widely used as ornamental shrubs in gardens and their hybrids with brilliant horticultural traits are increasingly popular worldwide. In this study, genome sizes of fourteen Viburnum species were estimated by using flow cytometry. Genome size for diploids ranged from 255pg in Vschensianum to 426pg in Vmacrocephalum form. keteleeri. And Vmacrocephalum form. keteleeri also had a more asymmetrical karyotype which may reflect its breeding history. The genome size of tetraploid species Vodoratissimum (762pg) was unusually more than twice as big as those of other diploid species, which revealed that polyploidization in this genus may be not remote in its evolution. These results lay a foundation for further research in the cytogenetics and taxonomy of Viburnum and provide useful insight into future hybridization efforts for this genus.

Key words: Viburnum, Flow cytometry, Genome size, Variation, Chromosome

中图分类号:

Q 75

张宁宁, 杨静, 孙卫邦. 利用流式细胞仪测定荚蒾属植物的基因组大小[J]. Plant Diversity, 2014, 36(06): 730-736.

ZHANG Ning-Ning, YANG Jing, SUN Wei-Bang. Genome Size Estimation of Viburnum (Adoxaceae) Species by Using Flow Cytometry*[J]. Plant Diversity, 2014, 36(06): 730-736.

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利用流式细胞仪测定荚蒾属植物的基因组大小

Genome Size Estimation of Viburnum (Adoxaceae) Species by Using Flow Cytometry*

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