Seed dormancy, germination and storage behavior of Magnolia sinica, a plant species with extremely small populations of Magnoliaceae

doi:10.1016/j.pld.2021.06.009

Plant Diversity ›› 2022, Vol. 44 ›› Issue (01): 94-100.DOI: 10.1016/j.pld.2021.06.009

Seed dormancy, germination and storage behavior of Magnolia sinica, a plant species with extremely small populations of Magnoliaceae

Liang Lin^a, Lei Cai^b, Lei Fan^a, Jun-Chao Ma^a, Xiang-Yun Yang^a, Xiao-Jian Hu^a

a The Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China;
b Yunnan Key Laboratory for Integrative Conservation of Plant Species with Extremely Small Populations, Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China

收稿日期:2020-11-19 修回日期:2021-06-29 出版日期:2022-02-25 发布日期:2022-03-12
通讯作者: Xiao-Jian Hu
基金资助:
We thank Prof. Hugh W. Pritchard for his great help on revision of the manuscript. We also thank Prof. Wei-Qi Li for his critical comments on the manuscript. We also would like to thank Yun-Gang Guo, Juan Yang, Cheng Liu and Mei-Juan Li of the Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences for their help in this study. This study was supported by the Shandong Provincial Agricultural Elite Varieties Project ‘Research on key techniques for conservation of wild forest species in germplasm bank’ (grant no.2019LZGC01801); the Biological Resources Programme, Chinese Academy of Sciences (KFJ-BRP-017-75); the Science & Technology Basic Resources Investigation Program of China (grant no. 2017FY100100); the open research project of "Cross-Cooperative Team" of the Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences and the National Natural Science Foundation of China (grant no. 31500272). This study was conducted using the facilities of the Large Research Infrastructures User Service Platform, Chinese Academy of Sciences.

Seed dormancy, germination and storage behavior of Magnolia sinica, a plant species with extremely small populations of Magnoliaceae

Liang Lin^a, Lei Cai^b, Lei Fan^a, Jun-Chao Ma^a, Xiang-Yun Yang^a, Xiao-Jian Hu^a

a The Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China;
b Yunnan Key Laboratory for Integrative Conservation of Plant Species with Extremely Small Populations, Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China

Received:2020-11-19 Revised:2021-06-29 Online:2022-02-25 Published:2022-03-12
Contact: Xiao-Jian Hu
Supported by:
We thank Prof. Hugh W. Pritchard for his great help on revision of the manuscript. We also thank Prof. Wei-Qi Li for his critical comments on the manuscript. We also would like to thank Yun-Gang Guo, Juan Yang, Cheng Liu and Mei-Juan Li of the Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences for their help in this study. This study was supported by the Shandong Provincial Agricultural Elite Varieties Project ‘Research on key techniques for conservation of wild forest species in germplasm bank’ (grant no.2019LZGC01801); the Biological Resources Programme, Chinese Academy of Sciences (KFJ-BRP-017-75); the Science & Technology Basic Resources Investigation Program of China (grant no. 2017FY100100); the open research project of "Cross-Cooperative Team" of the Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences and the National Natural Science Foundation of China (grant no. 31500272). This study was conducted using the facilities of the Large Research Infrastructures User Service Platform, Chinese Academy of Sciences.

摘要/Abstract

摘要： Magnolia sinica is one of the most endangered Magnoliaceae species in China. Seed biology information concerning its long-term ex situ conservation and utilization is insufficient. This study investigated dormancy status, germination requirements and storage behavior of M. sinica. Freshly matured seeds germinated to ca. 86.5% at 25/15℃ but poorly at 30℃; GA₃ and moist chilling promoted germination significantly at 20℃. Embryos grew at temperatures (alternating or constant) between 20℃ and 25℃, but not at 5℃ or 30℃. Our results indicate that M. sinica seeds possibly have non-deep simple morphophysiological dormancy (MPD). Seeds survived desiccation to 9.27% and 4.85% moisture content (MC) as well as a further 6-month storage at -20℃ and in liquid nitrogen, including recovery in vitro as excised embryos. The established protocol ensured that at least 58% of seedlings were obtained after both cold storage and cryopreservation. These results indicate that both conventional seed banking and cryopreservation have potential as long-term ex situ conservation methods, although further optimized approaches are recommended for this critically endangered magnolia species.

关键词: Magnolia sinica, Seed, Dormancy, Storage behavior, Cryopreservation, Excised embryo

Abstract: Magnolia sinica is one of the most endangered Magnoliaceae species in China. Seed biology information concerning its long-term ex situ conservation and utilization is insufficient. This study investigated dormancy status, germination requirements and storage behavior of M. sinica. Freshly matured seeds germinated to ca. 86.5% at 25/15℃ but poorly at 30℃; GA₃ and moist chilling promoted germination significantly at 20℃. Embryos grew at temperatures (alternating or constant) between 20℃ and 25℃, but not at 5℃ or 30℃. Our results indicate that M. sinica seeds possibly have non-deep simple morphophysiological dormancy (MPD). Seeds survived desiccation to 9.27% and 4.85% moisture content (MC) as well as a further 6-month storage at -20℃ and in liquid nitrogen, including recovery in vitro as excised embryos. The established protocol ensured that at least 58% of seedlings were obtained after both cold storage and cryopreservation. These results indicate that both conventional seed banking and cryopreservation have potential as long-term ex situ conservation methods, although further optimized approaches are recommended for this critically endangered magnolia species.

Key words: Magnolia sinica, Seed, Dormancy, Storage behavior, Cryopreservation, Excised embryo

Liang Lin, Lei Cai, Lei Fan, Jun-Chao Ma, Xiang-Yun Yang, Xiao-Jian Hu. Seed dormancy, germination and storage behavior of Magnolia sinica, a plant species with extremely small populations of Magnoliaceae[J]. Plant Diversity, 2022, 44(01): 94-100.

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Seed dormancy, germination and storage behavior of Magnolia sinica, a plant species with extremely small populations of Magnoliaceae

Seed dormancy, germination and storage behavior of Magnolia sinica, a plant species with extremely small populations of Magnoliaceae

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