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Plant Diversity ›› 2008, Vol. 30 ›› Issue (6): 699-705.DOI: 10.3724 SP.J.1143.2008.08043

• 研究论文 • 上一篇    下一篇

高表达水稻WRKY72 基因影响拟南芥生长素信号传导

宋 钰1 , 2 , 刘冬梅1 , 2 , 余迪求1   

  1. 1 中国科学院西双版纳热带植物园植物分子实验室, 云南昆明 650223;
    2 中国科学院研究生院, 北京 100049
  • 收稿日期:2008-03-21 出版日期:2008-12-25 发布日期:2008-12-25
  • 通讯作者: 余迪求

Overexpression of OsWRKY72 Gene Causes Auxin Signaling Passway in Arabidopsis

SONG Yu1 , 2 , LIU Dong-Mei1 , 2 , YU Di-Qiu1   

  1. 1 Laboratory of Plant Molecular Biology , Xishuangbanna Tropical Botanical Garden , Chinese Academy of Sciences, Kunming 650223 , China ; 2 Graduate University of Chinese Academy of Sciences , Beijing 100049
  • Received:2008-03-21 Online:2008-12-25 Published:2008-12-25
  • Contact: YU Di-Qiu1

摘要: 植物转录调控因子WRKY 基因家族是一个拥有众多成员的超家族, 功能涵盖了植物生长发育的控制与抗病耐逆的调节。我们主要分析了OsWRKY72 基因在外源植物拟南芥中的生物学功能。通过转基因拟南芥( Arabidopsis thaliana) 的遗传学研究发现外源高表达该基因不单明显地抑制转基因植株的顶端优势, 增强植株侧枝的生长, 还改变了转基因植株叶片和角果的发育。进一步分析证实, 高表达OsWRKY72 基因所导致转基因拟南芥植株的表型和其它生理现象都与生长素信号通路改变所导致的表型和生理变化极其相近。这些结果说明OsWRKY72 基因在外源植物拟南芥体内高表达后很可能改变了其正常的生长素信号通路。

关键词: OsWRKY72, 顶端优势, 生长素

Abstract: The transcription factors WRKY protein superfamily are widely presented in plants . It is well known that a majority of the plant biological processes are modulated by WRKY transcription factors . And here we focused on OsWRKY72. Genetic analysis showed the apical dominance of 35S:OsWRKY72 transgenic Arabidopsis thaliana was repressed and the transgenic plants had significantly more shoot branches comparing with the control plants . Besides the reduced apical dominance, the phenotypes , including curled leaves and short fruits in transgenic Arabidopsis, were similar to those of the characteristic mutants deficiency in auxin singal transduction. These results suggested that OsWRKY72 played an important role in the auxin signaling pathway.

Key words: OsWRKY72

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