梵净山尖叶拟船叶藓的遗传多样性分析

Plant Diversity ›› 2008, Vol. 30 ›› Issue (02): 168-172.

梵净山尖叶拟船叶藓的遗传多样性分析

李朝阳, 李菁, 田向荣, 姜业芳, 陈军

收稿日期:2007-06-25 出版日期:2008-04-25 发布日期:2008-04-25
通讯作者: 李朝阳

Analysis on Genetic Diversity of Dolichomitriopsis diversiformis(Lembophyllaceae) in Fanjing Mountain

LI Zhao-Yang, LI Jing , TIAN Xiang-Rong, JIANG Ye-Fang, CHEN Jun

Received:2007-06-25 Online:2008-04-25 Published:2008-04-25
Contact: LI Zhao-Yang

摘要/Abstract

摘要： 采用RAPD 技术, 选取10 个引物对梵净山的尖叶拟船叶藓( Dolichomitriopsis diversiformis) 自然分布区的南、北坡14 个居群153 个个体的总DNA 进行了扩增, 共得到196 个位点。统计分析表明: (1) 梵净山尖叶拟船叶藓在物种水平有较高的遗传多样性, 居群水平的遗传多样性相对较低。(2) 该藓种遗传多样性高低与海拔高度无关, 但南坡的多样性水平略高于北坡。( 3) 遗传多样性的63 .29%发生在居群间, 只有36.71%发生在居群内。研究结果提示尖叶拟船叶藓的遗传多样性受小生境的影响较大, 遗传漂变和环境适应可能是影响居群分化的主要原因。

关键词: 梵净山, 尖叶拟船叶藓, RAPD, 遗传多样性

Abstract: Dolichomitriopsis diversiformis is the only Dolichomitriopsis species in China, and is the member of Lembophyllaceae. Ten random primer pairs were selected to amplified 153 individuals from 14 D. diversiformis populations from both northern and southern slops of Fanjing Mountains by using RAPD methods, and 196 sites were derived . Results suggested that (1) D. diversiformis from FanjingMount kept a high level genetic diversity, and the genetic diversity is relative low at the population level ; (2) genetic diversity have no relationship with altitude, but populations on southern slope have a little higher diversity than that on the northern slope; (3) 63.29% genetic diversity exist among populations, and only 36.71% within population. These results suggested that small habitat was the major impact factors of D. diversiformis genetic diversity, genetic drift and habit adaption should be the prominent reason of population differentiation .

Key words: Fanjing mountain

中图分类号:

Q 16

李朝阳, 李菁, 田向荣, 姜业芳, 陈军. 梵净山尖叶拟船叶藓的遗传多样性分析[J]. Plant Diversity, 2008, 30(02): 168-172.

LI Zhao-Yang, LI Jing , TIAN Xiang-Rong, JIANG Ye-Fang, CHEN Jun. Analysis on Genetic Diversity of Dolichomitriopsis diversiformis(Lembophyllaceae) in Fanjing Mountain[J]. Plant Diversity, 2008, 30(02): 168-172.

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