[an error occurred while processing this directive]

Plant Diversity ›› 2015, Vol. 37 ›› Issue (06): 871-880.DOI: 10.7677/ynzwyj201515048

• 研究论文 • 上一篇    下一篇


 余亚莹1, 邵高能1, 圣忠华1, 蒋汉伟2, 贺记外1, 孙园园2, 蔡怡聪1, 胡培松1**, 唐绍清1   

  1. 1 中国水稻研究所/农业部水稻生物学与遗传育种重点实验室/水稻生物学国家重点实验室,
    杭州310006;2 杭州师范大学,杭州310006
  • 收稿日期:2015-03-19 出版日期:2015-11-25 发布日期:2015-05-12
  • 基金资助:

    国家自然科学基金国际合作项目 (31161140348),浙江自然科学基金 (LY14C130011)

Genetic Diversity of Global Aromatic Rice Varieties

 YU  Ya-Ying-1, SHAO  Gao-Neng-1, SHENG  Zhong-Hua-1, JIANG  Han-Wei-2, HE  Ji-Wai-1, SUN  Yuan-Yuan-2, CA  Yi-Cong-1, HU  Pei-Song-1**, TANG  Shao-Qing-1   

  1. 1 State Key Laboratory of Rice Biology, Key Laboratory of Rice Biology and Breeding of Ministry of Agriculture, China National
    Rice Research Institute, Hangzhou 310006, China; 2 Hangzhou Normal University, Hangzhou 310006, China
  • Received:2015-03-19 Online:2015-11-25 Published:2015-05-12
  • Supported by:

    国家自然科学基金国际合作项目 (31161140348),浙江自然科学基金 (LY14C130011)


收集来源于16个国家和地区的345份香稻和89份非香材料开展了遗传多样性研究。利用分布于12条染色体上77对SSR标记,共检测出573个等位基因位点,多态信息含量 (PIC) 变幅为0090~0845,平均为0516;基因多样性 (GD) 变幅为0091~0859,平均为0573;主效等位基因频率 (MAF) 平均为0540,变幅从0251~0953。聚类分析和群体结构分析将434份材料分成3个亚群,亚群与亚群间FST平均为-0116,变幅从-0623~0494;据Nei’s方法计算的亚群间遗传距离从0207~0355;各亚群内的主效等位基因频率为0408~0746,基因多样性变幅为0354~0699,多态信息含量则从0320~0658。通过对18个水稻品种中的6个线粒体基因测序结果发现5个基因内不存在核苷酸的差异,仅Mit4中包含了3个SNP的两种基因型,可以作为反应籼粳分化与香味基因进化的重要功能标记。本研究较为系统地反映了不同香稻材料间以及与非香稻资源间的亲缘关系,为进一步了解国内外香稻种质资源和优质香稻育种提供了重要的参考依据。

关键词: 香稻, SSR标记, 遗传多样性, 聚类分析


Genetic diversity of 434 rice accessions collected from 16 countries, including 345 fragrance rice varieties and 89 nonfragrance rice varieties, have been analyzed. A total of 573 alleles were detected by using 77 simple sequence repeats (SSR) primer pairs covering all rice 12 chromosomes. The value of allelic polymorphism information content (PIC) ranged from 0090 to 0845, with an average of 0516 per locus; Gene diversity (GD) varied from 0091 to 0859, with an average of 0573; The mean value of major allele frequencies (MAF) was 0540, covering from 0251 to 0953. In addition, 434 rice accessions are divided into three subpopulations by cluster and population structure analysis, and FST between subpopulations showed a mean of -0116, ranging from -0623 to 0494; The score of genetic distance calculated by Nei′s method appeared from 0207 to 0355. Major allele frequencies within each subpopulation distributed from 0408 to 0746, and gene diversity level from 0354 to 0699, while PIC from 0320 to 0658. Sequencing 6 mitochondrion genes in 18 rice varieties exhibited no different in 5 genes, whereas Mit4 contains a 3 SNPs in the gene body, which acts as an important marker to understanding the relationship between Indica/Japonica differentiation and the evolution of fragrant gene. Finally, genetic diversity and mitochondrion gene sequencing would help to know about the origin of fragrant resource and benefit rice breeding.

Key words: Aromatic rice, SSR markers, Genetic diversity, Cluster analysis