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Plant Diversity ›› 2015, Vol. 37 ›› Issue (2): 141-144.DOI: 10.7677/ynzwyj201514063

• 研究论文 • 上一篇    下一篇

南天竹微卫星标记开发及特性分析

 呼慧丽, 李波, 胡菀, 孙志霞, 张志勇, 范邓妹   

  1. 江西农业大学亚热带生物多样性实验室,南昌330045
  • 收稿日期:2014-04-10 出版日期:2015-03-25 发布日期:2014-07-20
  • 基金资助:

    National Natural Science Foundation of China (grant nos. 31160043 and 31160082) and National Science and Technology Support Program (2012BAC11B02)

Development and Characterization of Microsatellite Loci for Heavenly Bamboo (Nandina domestica)

 HU  Hui-Li, LI  Bo, HU  Wan, SUN  Zhi-Xia, ZHANG  Zhi-Yong, FAN  Deng-Mei   

  1. Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang 330045, China
  • Received:2014-04-10 Online:2015-03-25 Published:2014-07-20
  • Supported by:

    National Natural Science Foundation of China (grant nos. 31160043 and 31160082) and National Science and Technology Support Program (2012BAC11B02)

摘要:

:南天竹 (Nandina domestica) 是一种具有较高观赏价值的常绿灌木。本研究从南天竹基因组中开发和筛选出10个微卫星位点,能在该物种中进行稳定PCR扩增且具一定的多态性。采用4个南天竹群体24个个体进行检测发现,每个位点的等位基因数为2~6,期望杂合度和表观杂合度分别为0153~0778和0~1。本研究结果为后续对南天竹遗传多样性的研究打下了基础。另外,这10个位点在小檗科4个其他物种中也有一定的通用性。

关键词: 南天竹, 微卫星, 遗传多样性

Abstract:

We developed and characterized a total of 10 novel polymorphic microsatellite loci from the genome of an ornamental shrub, Nandina domestica. The number of alleles per locus in 24 individuals from four populations varied from 2 to 6. The expected and observed heterozygosity ranged from 0153 to 0778 and from 0 to 1, respectively. These markers will provide tools for future studies on population genetic diversity of Ndomestica. Additionally, most of our new markers could yield the amplification products in four other Berberidaceae species.

Key words: Nandina domestica, Microsatellite marker, Genetic diversity

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