PLDα1 介导ABA 调控的拟南芥主根伸长并参与根毛生长

doi:10.3724 SP.J.1143.2009.09066

Plant Diversity ›› 2009, Vol. 31 ›› Issue (04): 309-316.DOI: 10.3724 SP.J.1143.2009.09066

PLDα1 介导ABA 调控的拟南芥主根伸长并参与根毛生长

李婧, 章文华

南京农业大学生命科学学院, 江苏南京 210095

收稿日期:2009-04-02 出版日期:2009-08-25 发布日期:2009-08-25
通讯作者: 章文华

PLDα1 Modulated ABA-Mediated Main Root Elongation and Regulated Tip Growth of Root Hair in Arabidopsis

LI Jing, ZHANG Wen-Hua

College of Life Sciences, Nanjing Agricultural University, Nanjing 210095 , China

Received:2009-04-02 Online:2009-08-25 Published:2009-08-25
Contact: ZHANG Wen-Hua

摘要/Abstract

摘要：

探讨了磷脂酶Dα1 (PLDα1 ) 在ABA 抑制拟南芥主根伸长过程中的作用。PLDα1 基因突变体pldα1 主根伸长受ABA 抑制小于野生型(WT) ; 根系PLDα1 活性在ABA 处理下升高; 拟南芥根细胞原生质体中活性氧(ROS) 含量在ABA 处理下升高, 但是pldα1 升高小于WT; 根系NADPH 氧化酶活性在ABA 处理下升高, pldα1 升高小于WT, 外源加入10μmol L^{- 1} PA (磷脂酸, PLD 水解产物) 后, 前者活性显著升高; 外源加入H₂O₂可诱导WT 和pldα1 主根伸长都受到抑制, 且二者差异不明显。结果表明, PLDα1 产生的PA 通过激活NADPH 氧化酶产生ROS 介导ABA 调控的拟南芥主根伸长过程。此外, 初步探讨了PLDα1 在拟南芥根毛尖端生长中的作用: pldα1 突变体根毛长度小于WT, 根毛尖端ROS 和Ca²⁺ 浓度低于WT。

关键词: 拟南芥, ABA, 根, PLDα1, PA, ROS, Ca² ⁺ , 根毛

Abstract:

The role of PLDα1 in ABA inhibition of main root elongation in Arabidopsis was studied . When the seedlings were treated with ABA, elongation of main root of wild- type (WT) was inhibited more obviously than that of pldα1. In main
root protoplasts, the activity of both PLDα1 and NADPH oxidase increased under ABA treatment, and the NADPH oxidase activity increased much more in WT than in pldα1. When 10μmol L^{- 1} PA was added into the growth medium, the NADPH oxidase activity increased in pldα1 protoplasts. The concentration of ROS in Arabidopsis root protoplasts increased more quickly in WT than in pldα1 after ABA addition. When H2O2 was added into the culture medium, main root elongation was inhibited in both genotypes. All these results suggested that, PA produced by PLDα1 acted as a regulator in ABAmediated
root elongation in Arabidopsis via NADPH oxidase-produced ROS. In addition, root hair of pldα1 was shorter than that of WT. On the tip of root hair, pldα1 had lower concentration of ROS and Ca²⁺ than WT .

Key words: Arabidopsis, Root hair

中图分类号:

Q 945

李婧, 章文华 .

PLDα1 介导ABA 调控的拟南芥主根伸长并参与根毛生长

[J]. Plant Diversity, 2009, 31(04): 309-316.

LI Jing, ZHANG Wen-Hua. PLDα1 Modulated ABA-Mediated Main Root Elongation and Regulated Tip Growth of Root Hair in Arabidopsis[J]. Plant Diversity, 2009, 31(04): 309-316.

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PLDα1 介导ABA 调控的拟南芥主根伸长并参与根毛生长

PLDα1 Modulated ABA-Mediated Main Root Elongation and Regulated Tip Growth of Root Hair in Arabidopsis

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