Landscape genetics reveals inbreeding and genetic bottlenecks in the extremely rare short-globose cacti Mammillaria pectinifera (Cactaceae) as a result of habitat fragmentation

doi:10.1016/j.pld.2016.09.005

Plant Diversity ›› 2017, Vol. 39 ›› Issue (01): 13-19.DOI: 10.1016/j.pld.2016.09.005

Landscape genetics reveals inbreeding and genetic bottlenecks in the extremely rare short-globose cacti Mammillaria pectinifera (Cactaceae) as a result of habitat fragmentation

Reyna Maya-García^a, Santiago Arizaga^b, Pablo Cuevas-Reyes^a, Juan Manuel Pe?aloza-Ramírez^b, Víctor Rocha Ramírez^b, Ken Oyama^b

a. Facultad de Biología, Universidad Michoacana de San Nicolás de Hidalgo, Mexico;
b. Escuela Nacional de Estudios Superiores Unidad Morelia, UNAM, Antigua Carretera a Pátzcuaro 8701, Colonia Ex-Hacienda de San José de la Huerta, 58190, Morelia, Michoacán, Mexico

收稿日期:2016-04-29 修回日期:2016-09-06 出版日期:2017-02-25 发布日期:2021-11-05
通讯作者: Juan Manuel Pe?aloza-Ramírez
基金资助:
We thank to D. Lugo-Aquino, N. Pérez-Nasser and Ana Luisa Albarrán-Lara for technical assistance and suggested improvements to the manuscript. This project was supported by SDI-UNAM (Secretaria de Desarrollo Institucional, Universidad Nacional Autónoma de México, Octavo piso Torre de Rectoría, Ciudad Universitaria).

Landscape genetics reveals inbreeding and genetic bottlenecks in the extremely rare short-globose cacti Mammillaria pectinifera (Cactaceae) as a result of habitat fragmentation

Reyna Maya-García^a, Santiago Arizaga^b, Pablo Cuevas-Reyes^a, Juan Manuel Pe?aloza-Ramírez^b, Víctor Rocha Ramírez^b, Ken Oyama^b

a. Facultad de Biología, Universidad Michoacana de San Nicolás de Hidalgo, Mexico;
b. Escuela Nacional de Estudios Superiores Unidad Morelia, UNAM, Antigua Carretera a Pátzcuaro 8701, Colonia Ex-Hacienda de San José de la Huerta, 58190, Morelia, Michoacán, Mexico

Received:2016-04-29 Revised:2016-09-06 Online:2017-02-25 Published:2021-11-05
Contact: Juan Manuel Pe?aloza-Ramírez
Supported by:
We thank to D. Lugo-Aquino, N. Pérez-Nasser and Ana Luisa Albarrán-Lara for technical assistance and suggested improvements to the manuscript. This project was supported by SDI-UNAM (Secretaria de Desarrollo Institucional, Universidad Nacional Autónoma de México, Octavo piso Torre de Rectoría, Ciudad Universitaria).

摘要/Abstract

摘要： Mammillaria pectinifera is an endemic, short-globose cactus species, included in the IUCN list as a threatened species with only 18 remaining populations in the Tehuacán-Cuicatlán Valley in central Mexico. We evaluated the population genetic diversity and structure, connectivity, recent bottlenecks and population size, using nuclear microsatellites. M. pectinifera showed high genetic diversity but some evidence of heterozygote deficiency (F), recent bottlenecks in some populations and reductions in population size. Also, we found low population genetic differentiation and high values of connectivity for M. pectinifera, as the result of historical events of gene flow through pollen and seed dispersal. M. pectinifera occurs in sites with some degree of disturbance leading to the isolation of its populations and decreasing the levels of gene flow among them. Excessive deforestation also changes the original vegetation damaging the natural habitats. This species will become extinct if it is not properly preserved. Furthermore, this species has some ecological features that make them more vulnerable to disturbance such as a very low growth rates and long life cycles. We suggest in situ conservation to prevent the decrease of population sizes and loss of genetic diversity in the natural protected areas such as the Tehuacán-Cuicatlán Biosphere Reserve. In addition, a long-term ex situ conservation program is need to construct seed banks, and optimize seed germination and plant establishment protocols that restore disturbed habitats. Furthermore, creating a supply of living plants for trade is critical to avoid further extraction of plants from nature.

关键词: Mammillaria pectinifera, Nuclear SSR, Genetic diversity, Recent bottlenecks, Effective population size

Abstract: Mammillaria pectinifera is an endemic, short-globose cactus species, included in the IUCN list as a threatened species with only 18 remaining populations in the Tehuacán-Cuicatlán Valley in central Mexico. We evaluated the population genetic diversity and structure, connectivity, recent bottlenecks and population size, using nuclear microsatellites. M. pectinifera showed high genetic diversity but some evidence of heterozygote deficiency (F), recent bottlenecks in some populations and reductions in population size. Also, we found low population genetic differentiation and high values of connectivity for M. pectinifera, as the result of historical events of gene flow through pollen and seed dispersal. M. pectinifera occurs in sites with some degree of disturbance leading to the isolation of its populations and decreasing the levels of gene flow among them. Excessive deforestation also changes the original vegetation damaging the natural habitats. This species will become extinct if it is not properly preserved. Furthermore, this species has some ecological features that make them more vulnerable to disturbance such as a very low growth rates and long life cycles. We suggest in situ conservation to prevent the decrease of population sizes and loss of genetic diversity in the natural protected areas such as the Tehuacán-Cuicatlán Biosphere Reserve. In addition, a long-term ex situ conservation program is need to construct seed banks, and optimize seed germination and plant establishment protocols that restore disturbed habitats. Furthermore, creating a supply of living plants for trade is critical to avoid further extraction of plants from nature.

Key words: Mammillaria pectinifera, Nuclear SSR, Genetic diversity, Recent bottlenecks, Effective population size

Reyna Maya-García, Santiago Arizaga, Pablo Cuevas-Reyes, Juan Manuel Pe?aloza-Ramírez, Víctor Rocha Ramírez, Ken Oyama. Landscape genetics reveals inbreeding and genetic bottlenecks in the extremely rare short-globose cacti Mammillaria pectinifera (Cactaceae) as a result of habitat fragmentation[J]. Plant Diversity, 2017, 39(01): 13-19.

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Landscape genetics reveals inbreeding and genetic bottlenecks in the extremely rare short-globose cacti Mammillaria pectinifera (Cactaceae) as a result of habitat fragmentation

Landscape genetics reveals inbreeding and genetic bottlenecks in the extremely rare short-globose cacti Mammillaria pectinifera (Cactaceae) as a result of habitat fragmentation

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