NaPDR1 and NaPDR1-like are essential for the resistance of Nicotiana attenuata against fungal pathogen Alternaria alternata

doi:10.1016/j.pld.2018.01.001

Plant Diversity ›› 2018, Vol. 40 ›› Issue (02): 68-73.DOI: 10.1016/j.pld.2018.01.001

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NaPDR1 and NaPDR1-like are essential for the resistance of Nicotiana attenuata against fungal pathogen Alternaria alternata

Zhen Xu^a,b, Na Song^a, Lan Ma^a, Dunhuang Fang^c, Jinsong Wu^a

a Key Laboratory of Economic Plants and Biotechnology, Yunnan Key Laboratory for Research and Development of Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Lanhei Road 132, 650201, Kunming, China;
b College of Life Science, University of Yunnan, Kunming, 650091, China;
c Yunnan Academy of Tobacco Agricultural Sciences, Kunming, 650021, China

Received:2017-12-09 Revised:2018-01-08 Online:2018-04-25 Published:2018-07-25
Contact: Dunhuang Fang, Jinsong Wu
Supported by:
This project is supported by the NSFC (Grant No. 31670262), Key Project of Applied Basic Research Program of Yunnan (Grant No. 2014FA040), and 100-Oversea-Top-Talents Recruitment plan of Yunnan to Jinsong Wu, Project of Applied Basic Research Program of Yunnan (Grant No. 2017FB048) to Lan Ma, and founding (Grant No. 110201603008) to Dunhuang Fang.

Abstract

Abstract:

Pleiotropic drug resistance (PDR) transporters are widely distributed membrane proteins catalyzing the export or import of a diverse array of molecules,and are involved in many plant responses to biotic and abiotic stresses.However,it is unclear whether PDRs are involved in Nicotiana attenuata resistance to the necrotic fungal pathogen Alternaria alternata.In this study,transcriptional levels of both NaPDR1 and NaPDR1-like were highly induced in N.attenuata leaves after A.alternata inoculation.Interestingly, silencing NaPDR1 or NaPDR1-like individually had little effect on N.attenuata resistance to A.alternata; however,when both genes were co-silenced plants became highly susceptible to the fungus,which was associated with elevated JA and ethylene responses.Neither NaPDR1 nor NaPDR1-like was significantly elicited by exogenous treatment with methyl jasmonate (MeJA),whereas both were highly induced by ethylene.The elicitation levels of both genes by A.alternata were significantly reduced in plants with impaired JA or ethylene signaling pathways.Thus,we conclude that both NaPDR1 and NaPDR1-like function redundantly to confer resistance against A.alternata in N.attenuata,and the elicitation of the transcripts of both genes by the fungus is partially dependent on ethylene and jasmonate signaling.

Key words: ABC transporter, Ethylene, Jasmonate, Plant resistance, Pleiotropic drug resistance

Zhen Xu, Na Song, Lan Ma, Dunhuang Fang, Jinsong Wu. NaPDR1 and NaPDR1-like are essential for the resistance of Nicotiana attenuata against fungal pathogen Alternaria alternata[J]. Plant Diversity, 2018, 40(02): 68-73.

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NaPDR1 and NaPDR1-like are essential for the resistance of Nicotiana attenuata against fungal pathogen Alternaria alternata

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