Plant Diversity ›› 2013, Vol. 35 ›› Issue (2): 149-157.DOI: 10.7677/ynzwyj201312102

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The Effects of Phospholipase Dδ Suppression on the Responsesof ROS and Hormones to Artificial Leaf Senescence in Arabidopsis thanliana

 GU  Yan-Xia-1、2, DAO  Fa-Qing-1, LI  Wei-Qi-1   

  1. 1 The Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
    2 University of Chinese Academy of Sciences, Beijing 100039, China
  • Received:2012-08-06 Online:2013-03-25 Published:2012-10-09
  • Supported by:

    Grants from the National Basic Research Program of China (31070262), Fund of State Key Laboratory of Phytochemistry and Plant Resources in West China (O97C0211Z1)


The reactive oxygen species (ROS) and hormones can act as an important internal or external factor influencing plant senescence. In the present study, we found that suppression of phospholipase Dδ (PLDδ) attenuated ROS production during abscisic acid (ABA) and ethylenepromoted leaf senescence. We also comparatively analyzed the content of endogenous hormones, ABA, methyl jasmonate (MeJA), indole3acetic acid (IAA), and total zeatin in detachment inducedsenescence leaves, exogenous ABA and ethylene promoted senescence in wild type and PLDδknockout (PLDδKO) Arabidopsis leaves. We found that the response patterns of the four endogenous hormones to the three senescence treatments were identical. In comparison with wild type, PLDδKO plants showed higher ZR and IAA levels and lower MeJA content under ABA and higher ZR and IAA levels and lower ABA and MeJA content under ethylene. The results suggested that these hormones might contribute to retarding ABA and ethylenepromoted senescence in PLDδknockout Arabidopsis.

Key words:  Phospholipase Dδ, ROS, Ethylene, Abscisic acid, Leaf

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