Large-scale interplant exchange of macromolecules between soybean and dodder under nutrient stresses

doi:10.1016/j.pld.2023.11.005

Plant Diversity ›› 2024, Vol. 46 ›› Issue (01): 116-125.DOI: 10.1016/j.pld.2023.11.005

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Large-scale interplant exchange of macromolecules between soybean and dodder under nutrient stresses

Jingxiong Zhang^a, Shalan Li^c, Wenxing Li^a,b, Zerui Feng^a,b, Shuhan Zhang^a,b, Xijie Zheng^a,b, Yuxing Xu^a, Guojing Shen^a,b, Man Zhao^a,b, Guoyan Cao^a, Xuna Wu^c, Jianqiang Wu^a,b

a. Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
b. CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, China;
c. State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan and Center for Life Science, School of Life Sciences, Yunnan University, Kunming 650500, China

Received:2023-10-15 Revised:2023-11-29 Online:2024-01-25 Published:2024-03-02
Contact: Xuna Wu,E-mail:xwu@ynu.edu.cn;Jianqiang Wu,E-mail:wujianqiang@mail.kib.ac.cn
Supported by:
We would like to express our gratitude to Dr. Wei Chang and Dr. Fei Li for their invaluable technical support. We thank the Service Center for Experimental Biotechnology at the Kunming Institute of Botany, CAS, for their assistance throughout the research. Furthermore, the Proteomics Facility of Life Science Center, Yunnan University, is thanked for their technical support and assistance in conducting the proteomic analysis. This work was supported by the National Natural Science Foundation of China (31970274 (J.W.), 32170272 (X.W.), 32100251 (J.Z.), 32000179 (Y.X.)), the Special Research Assistant of Chinese Academy of Sciences (J.Z. and Y.X.), China Postdoctoral Science Foundation (2022M713224 (J.Z.)), the Strategic Priority Research Program of Chinese Academy of Sciences (XDPB16 (J.W.)), the Yunnan Innovation Team Project (202105AE160013 (J.W.)), CAS “Light of West China” Program (G.S.), and Yunnan Revitalization Talent Support Program “Young Talents” Project (XDYC-QNRC-2022-0301 (J.Z.), XDYC-QNRC-2022-0001 (G.S.)), the General and Key Project of the Applied Basic Research Program of Yunnan (202001AS070021(J.W.)), Yunnan Fundamental Research Projects-General Project (202101AT070457 (S.L.)), and Yunnan Fundamental Research Projects-Youth Talent Project (202101AU070021(S.L.)).

Abstract

Abstract: Parasitic plants and their hosts communicate through haustorial connections. Nutrient deficiency is a common stress for plants, yet little is known about whether and how host plants and parasites communicate during adaptation to such nutrient stresses. In this study, we used transcriptomics and proteomics to analyze how soybean (Glycine max) and its parasitizing dodder (Cuscuta australis) respond to nitrate and phosphate deficiency (-N and -P). After -N and -P treatment, the soybean and dodder plants exhibited substantial changes of transcriptome and proteome, although soybean plants showed very few transcriptional responses to -P and dodder did not show any transcriptional changes to either -N or -P. Importantly, large-scale interplant transport of mRNAs and proteins was detected. Although the mobile mRNAs only comprised at most 0.2% of the transcriptomes, the foreign mobile proteins could reach 6.8% of the total proteins, suggesting that proteins may be the major forms of interplant communications. Furthermore, the interplant mobility of macromolecules was specifically affected by the nutrient regimes and the transport of these macromolecules was very likely independently regulated. This study provides new insight into the communication between host plants and parasites under stress conditions.

Key words: Cuscuta, Mobile molecules, Nutrient deficiency, Host plant-parasitic plant interaction, Interplant transport, Systemic signaling

Jingxiong Zhang, Shalan Li, Wenxing Li, Zerui Feng, Shuhan Zhang, Xijie Zheng, Yuxing Xu, Guojing Shen, Man Zhao, Guoyan Cao, Xuna Wu, Jianqiang Wu. Large-scale interplant exchange of macromolecules between soybean and dodder under nutrient stresses[J]. Plant Diversity, 2024, 46(01): 116-125.

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Large-scale interplant exchange of macromolecules between soybean and dodder under nutrient stresses

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