Plant Diversity ›› 2010, Vol. 32 ›› Issue (02): 147-150.DOI: 10.3724/SP.J.1143.2010.09204
• Articles •
LI YanYan1, 2, CAO JiaShu2
The rapid alkalinization factor (RALF) gene is a new found plant polypeptide signal molecule, wide spreading in higher plants. In this study BcRALF was cloned from Brassica campestris sspchinensis Makino varparachinensis based on BcMF14 (GenBank accession number EF523516) from Bcampestris ssp. chinensis var. communis cv. Aijiaohuang. The sequence of this gene was 273 bp (GU086228) and was identified as a rapid alkalization factor gene according to its high identity with Boleracea var botrytis BoRALF1 and Arabidopsis thaliana RALFL9. Protein characteristics and sequence structure were predicted, and moreover, many bioactive sites were found. The results showed that the characteristics of the BcRALF protein consistent with its category as a peptide signal molecule.
Brassica campestris sspchinensis varparachinensis
LI YanYan, , CAO JiaShu. Isolation and Molecular Characterization of RALFLike Gene BcRALF from Brassica campestris ssp.chinensis var.parachinensis[J]. Plant Diversity, 2010, 32(02): 147-150.
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