Ultracytochemical Localization of Calcium during Embryo Sac Development in Phaius tankervilliae(Orchidaceae)

doi:10.3724/SP.J.1143.2010.10139

Abstract

Abstract:

Ultracytochemical localization of calcium in embryo sac of Phaius tankervilliae(Aiton) Bl. was carried out using potassium pyroantimonate precipitation method. Results of Ca2+ distribution during embryo sac development are as following: (1) Megasporocyte stage: There is no Ca2+ precipitation in the megasporocyte, nucellar cell and micropyle; (2) Functional megaspore stage: Small particles of Ca2+ precipitates appear on the embryo sac wall at the micropylar end, but no apparent Ca2+ can be found in the functional megaspore; (3) 4nucleate stage: There are significant increase of Ca2+ precipitation on the embryo sac wall and large grains of the precipitates in the micropyle, while a little starts to appear on the membrane of vacuole; (4) 8nucleate embryo sac stage: Ca2+ precipitation continuously increases on the embryo sac wall. Distribution of Ca2+ in embryo sac shows strong polarity. More Ca2+ precipitations are observed in the synergids and the egg cell than the antipodal cells. Accumulation of Ca2+ precipitation is achieved by (1) transferring Ca2+ to embryo sac through the plasmodesmata between the nucellar cells, which mainly occurred at the chalaza end before the embryo matured; (2) forming large amounts of small bubbles of Ca2+ precipitation, which can cross embryo sac wall to enter embryo sac.

Key words: Phaius tankervilliae, Embryo sac, Ultracytochemistry, Potassium antimonite technique, Ca2+

CLC Number:

Q 944

LI Dong-Mei-, WANG Y-Qin-, YIE Xiu-Lin. Ultracytochemical Localization of Calcium during Embryo Sac Development in Phaius tankervilliae(Orchidaceae)[J]. Plant Diversity, 2010, 32(6): 495-502.

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