Plant Diversity ›› 2011, Vol. 33 ›› Issue (5): 540-546.DOI: 10.3724/SP.J.1143.2011.11009

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Seed Storage Behavior of Formanodendron doichangensis (Fagaceae), a Rare and Endangered Plant

 CHEN  Hong-Ying-1、2, SUN  Wei-Bang-1, LI  Wei-Qi-1   

  1. 1 Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
    2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2011-01-11 Online:2011-10-25 Published:2011-04-08
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The seed storage behavior of Formanodendron doichangensis (Fagaceae) was still unclear due to the effect of empty seeds to the germination experiment caused by high abortion. Therefore there was lack of a best storage method for conserve this rare and endangered plant. In the present paper, the Selecta Gravity Separator and the Xray imagine systems were introduced to successfully enhance the portion of the full and healthy seeds from the high aborted seed lot, and exclude the effect of empty seeds to the germination experiment. Moreover, the desiccation tolerance and low temperature tolerance of F.doichangensis seeds were tested to identify the seed storage behavior and the best storage method for this endangered species was provided. The results showed that Selecta Gravity Separator could efficiently improved the percentage of full seeds in the seed lot from 47% (before clean) to 86% (after clean). F.doichangensis seeds kept high viability at 95% total germination similar to that of control seeds (92%), when experienced the extreme desiccation treatment (moisture content below 5%). After the storage at low temperature (-20℃) and liquid nitrogen (-196℃) for three months with low moisture content (<5%), the seed germination remained at 90% and 91% respectively. In conclusion, the mature F.doichangensis seeds were desiccation tolerant and low temperature tolerant, which exhibited orthodox seed storage behavior according to the international seed storage behavior classification, and could be kept in the seed bank storage facilities for long term conservation.

Key words:  Formanodendron doichangensis, Seed storage behavior, Desiccation tolerance, Low temperature tolerance

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