Plant Diversity ›› 2015, Vol. 37 ›› Issue (01): 63-70.DOI: 10.7677/ynzwyj201514025

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Storage Behavior Characteristics of Recalcitrant Podocarpus nagi Seeds*

 JI  MIng-Yue-1、2, ZHANG  Nan-1、2, YAN  Qi-1、2, WEN  Bin-1   

  1. 1 Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences,
    Mengla, Yunnan 666303, China; 2 University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2014-02-24 Online:2015-01-25 Published:2014-04-10
  • Supported by:

    国家自然科学基金 (31170626)


 This paper studied desiccation tolerance and optimal storage methods of Podocarpus nagi seeds and achieved the following results: Podocarpus nagi seeds were desiccation sensitive and recalcitrant, with an initial moisture content of (35 ± 07)% and a critical moisture content of (1686 ± 073)%; both moist storage and partiallydried storage can be applied to shortterm conservation of Podocarpus nagi seeds in practice, and storage at 4℃ produced better results than that at 15℃, but storage at subzero temperature was lethal no matter what seed moisture content was; In the partiallydried seed storage experiment, the undried seeds (the control) had no obvious viability decrease during 6months’ storage duration at 4℃ but seeds at critical moisture content had higher viability than those at other moisture contents after 9months storage; no matter what media moisture content was used, and whether stored at 4℃ or 15℃, 9months’ moist storage did not reduce Podocarpus nagi seed viability while seeds stored at 15℃ had significantly higher viability than those at 4℃ when storage expanded to 12 months, but some of these seeds were found germinating during 15℃ storage at the third month, and more and more germinated seeds there were as media moisture content and storage duration increasing; 2h flash drying to moisture content 7% let more than 90% excised embryos survive freezingthaw treatment and oneyear’s cryostorage made no difference to their post thaw survival, indicating that it is feasible to cryopreserve Podocarpus nagi. These findings can be referred to short and longterm conservation of recalcitrant seeds.

Key words: Recalcitrant seeds, Podocarpus nagi, Desiccation tolerance, Moist storage, Partially-dried storage, Cryopreservation, Germplasm conservation

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