Molecular and Functional Comparisons of Reactive Burst Oxygen Species Gene Family in Arabidopsis

doi:10.7677/ynzwyj201514148

Abstract

Abstract:

Reactive oxygen species (ROS) play a key role in cell signal transduction. They are involved in the regulation of growth, development, responses to abiotic or biotic stimuli and cell death. Recent studies identified respiratory burst oxidase homologues (RBOHs) as the key producers of ROS. The Arabidopsis genome contains 10 Rboh genes, that encode proteins with six transmembrane domains supporting two haem groups, FAD and NADPH hydrophilic domains in the Cterminal region and two calciumbinding domains (EFhand) in the Nterminal region. In the present study, we investigated patterns of transcription systematically in Arabidopsis plants. Comparisons among amino acid sequences of ten Rboh genes revealed high degrees of identity among entire amino acid sequences of three groups, suggesting that some members of Rboh family might have redundant functions. With the except of rbohC, none of the lossoffunction mutants was found to display distinct phenotypes, suggesting that RbohC might have a specific function in plant development.

Key words: NADPH oxidase, Respiratory burst oxidase homologues (Rboh) genes, Phylogenetic analysis, RT-PCR, Redundant function

CLC Number:

Q 75

SUN Xu-Dong. Molecular and Functional Comparisons of Reactive Burst Oxygen Species Gene Family in Arabidopsis[J]. Plant Diversity, 2015, 37(4): 463-471.

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