Plant Diversity ›› 2015, Vol. 37 ›› Issue (2): 157-167.doi: 10.7677/ynzwyj201514076

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Characterization of Genes Involved in Gibberellin Metabolism and Signaling Pathway in the Biofuel Plant Jatropha curcas

 GAO  Cong-Cong-1、2, NI  Jun-1、3, CHEN  Mao-Sheng-1、2, XU  Zeng-Fu-1   

  1. 1 Laboratory of Tropical Plant Resource Science, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences,
    Mengla, Yunnan 666303, China; 2 University of Chinese Academy of Sciences, Beijing 100049, China;
    3 School of Life Sciences, University of Science and Technology of China, Hefei 230026, China
  • Received:2014-05-13 Online:2015-03-25 Published:2014-07-10
  • Supported by:

    云南省高端科技人才引进计划项目 (2009CI123);云南省应用基础研究计划重点项目 (2011FA034) 和中国科学院“一三五”专项 (XTBG-T02) 的资助

Abstract:

Gibberellins (GAs) are essential phytohormones that control many aspects of plant development, including seed germination, stem elongation, leaf growth, flowering induction, development of glandular hairs, and pollen maturation. However, there are different mechanisms underlying GAregulated flowering in perennial woody plants and annual herb plants. To facilitate study about the role of GAs in the biofuel plant Jatropha curcas, we identified all genes involved in GA metabolism and signaling pathways. These genes include members of six gene families, ie., GA2oxidase (GA3ox), GA3oxidase (GA3ox), GA20oxidase (GA20ox), GA receptor GIBBERELLIN INSENSITIVE DWARF1 (GID1), DELLA growth inhibitors (DELLAs), and Fbox proteins, and two single genes SPINDLY (SPY) and EARLY FLOWERING1 (EL1). Jatropha homologs of genes from Arabidopsis and rice (Oryza sativa) were identified by blasting the genome and transcriptome database of Jatropha. Total 17 genes involved in GA metabolism and signaling pathway were identified from Jatropha, and were phylogenetically analyzed with homologs from Arabidopsis, rice, grape (Vitis vinifera), and castor bean (Ricinus communis). Our results showed that compared to Arabidopsis and rice, protein sequences of genes involved in GA metabolism and signaling pathways in Jatropha showed a higher similarity to those from castor bean and grape.

Key words: Gibberellin, Metabolism, Phytohormone signaling, Jatropha curcas, Phylogenetic analysis

CLC Number: 

  • Q 75