The Determination of Nuclear DNA Content (2Cvalue) on Some Representative Genus and Species of Magnoliaceae

doi:10.7677/ynzwyj201514164

Abstract

Abstract:

The plant nuclear DNA content (2Cvalue) is a principal characteristic parmeter to describe biodiversity of species，which has important significance on the study of plant resources. In this study, we choosed 23 species from 10 representative genera (Magnolia、Michelia、Manglietia、Liriodendron、Talauma、 Paramichelia、Tsoongiodendron、Manglietiastrum、Kmeria、Parakmeria) of Magnoliaceae in China. All samples were determined using a flow cytometry technique with a standard of Zea mays (545pg/2C). The amount of nuclear DNA among these species ranged from 325pg (317850Mbp) to 1361pg (1331058Mbp) for Pgrandiflora, Mofficinalis subsp. biloba respectively, and the coefficient of variation (CV) were less than 5%. The results of the study will not only provide references for determination of the nuclear DNA content of Magnoliaceae and other plants, but also lay the foundation for the utilization and conservation of Magnoliaceae plant resources.

Key words: Magnoliaceae, Flow cytometry, Nuclear DNA content (2Cvalue), Genome size

CLC Number:

Q 78

YE Lin-Jiang- , ZHANG Zhi-Rong-, SUN Zhi-Xia-, TIAN Shuang. The Determination of Nuclear DNA Content (2Cvalue) on Some Representative Genus and Species of Magnoliaceae[J]. Plant Diversity, 2015, 37(05): 605-610.

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