Diversity of Culturable Bacteria Associated with Tuber panzhihuanensePinus armandii Ectomycorrhizosphere Soil

doi:10.7677/ynzwyj201515032

Abstract

Abstract:

Truffles are edible hypogeous ectomycorrhizal fungi which have great economic importance for their organoleptic properties and have significant ecological interests for forestry. Although some new precious Chinese white truffle have been described constantly, the molecular mechanisms that control truffle body formation are largely unknown. It has been hypothesized that ectomycorrhizosphere soil communities may have influences on truffle production. Thus, isolation and molecular characterisation of culturable bacteria were carried out to investigate the bacteria diversity in mycorrhizosphere soil of Tuber panzhihuanensePinus armandii in this work. Sequencing results showed a significant presence mostly affiliated with Burkholderia was βProteobacteria (3098%). The second culturable fraction which dominated by Pseudomonas was γProteobacteria (288%) other isolates were mostly Phyllobacterium and Rhizobium, members of αProteobacteria (1467%), actinobacteria (125%) and Firmicutes (76%) represented by Arthrobacter and Bacillus, respectively. Chryseobacterium ureilyticum was the only bacterial strain belonging to Bacteroidetes. Similarities and differences of culturable bacterial community of ascocarps and ectomycorrhizosphere soil associated with Tuber were discussed.

Key words: Tuber panzhihuanense, Ectomycorrhizosphere soil, Bacteria diversity, 16S rDNA

CLC Number:

Q 938.1
Q 16

MO Shan-Ping- , ZHENG Yi-, SHANG Li-, LIU Pei-Gui-, WANG Ran-, XU Fu-Qiang. Diversity of Culturable Bacteria Associated with Tuber panzhihuanensePinus armandii Ectomycorrhizosphere Soil[J]. Plant Diversity, 2015, 37(06): 861-870.

References

Aspray TJ, Frey PK, Jones JE et al., 2006. Mycorrhization helper bacteria: a case of specificity for altering ectomycorrhiza architecture but not ectomycorrhiza formation［J］. Mycorrhiza, 16: 533—541
Allen MF, 1992. Mycorrhizal Functioning: An Integrative PlantFungal Process［M］. London: Chapmanand Hall
Barbieri E, Bertini L, Rossi I et al., 2005. New evidence for bacterial diversity in the ascoma of the ectomycorrhizal fungus Tuber borchii Vittad［J］. FEMS Microbiology Letters, 247: 23—35
Barbieri E, Guidi C, Bertaux J et al., 2007. Occurrence and diversity of bacterial communities in Tuber magnatum during truffle maturation［J］. Environmental Microbiology, 9: 2234—2246
Belimov AA, Safronova VI, Sergeyeva TA et al., 2001. Characterization of plant growth promoting rhizobacteria isolated from polluted soils and containing 1aminocyclopropane1carboxylate deaminase［J］. Canadian Journal of Microbiology, 47: 642—652
Bedini S, Bagnoli G, Sbrana C et al., 1999. Pseudomonads isolated from within fruit bodies of Tuber borchii are capable of producing biological control or phytostimulatory compounds in pure culture［J］. Symbiosis, 26: 223—236
Citterio B, Cardoni P, Potenza L et al., 1995. Isolation of bacteria from sporocarps of Tuber magnatum Pico, Tuber borchii Vittad. and Tuber maculatum Vitt.: identification and biochemical characterization［A］// Stocchi V, Bonfante P, Nuti M, Biotechnology of Ectomycorrhizae: Molecular Approaches［M］. New York: Plenum, 241—248
Citterio B, Malatesta M, Battistelli S et al., 2001. Possible involvement of Pseudomonas fluorescens and Bacillaceae in structural modifications of Tuber borchii fruitbodies［J］. Canadian Journal of Microbiology, 47: 264—268
Courty PE, Buée M, Diedhiou AG et al., 2010. The role of ectomycorrhizal communities in forest ecosystem processes: new perspectives and emerging concepts［J］. Soil Biology & Biochemistry, 42: 679—698
Dunstan WA, Malajczuk N, Dell B, 1998. Effects of bacteria on mycorrhizal development and growth of containergrown Eucalyptus diversicolor F. Muell. seedlings［J］. Plant and Soil, 201: 241—249
Founoune H, Duponnois R, B AM et al., 2001. Mycorrhiza Helper Bacteria stimulate ectomycorrhizal symbiosis of Acacia holosericea with Pisolithus alba［J］. New Phytologist, 153: 81—89
Founoune H, Duponnois R, Meyer JM et al., 2002. Interactions between ectomycorrhizal symbiosis and Fluorescent pseudomonads on Acacia holosericea: isolation of mycorrhiza helper bacteria (MHB) from a SoudanoSahelian soil［J］. FEMS Microbiol Ecology, 41: 37—46
Frey KP, Brule C, Garbaye J, 1997a. A proposed model for the mycorrhizahelper effect of Pseudomonas fluorescens BBc6 on the ectomycorrhizal system Laccaria bicolor S238NDouglas fir［C］. 4th International Workshop on Plant Growth Promoting Rhizobacteria, Sapporo, Japan
Frey P, Frey KP, Garbaye J et al., 1997b. Metabolic and genotypic fingerprinting of Fluorescent pseudomonads associated with the Douglas fir Laccaria bicolor mycorrhizosphere［J］. Apply Environment Microbiology, 63: 1852—1860
Fujii Y, Akihiro F, Syuntaro H, 2004. Rhizosphere soil method: a new bioassay to evaluate a llelopathy in the field［C］. Proceedings and selected papers of the fourth world congress on allelopathy, 490—492
Garbaye J, Bowen GD, 1987. Effect of different microflora on the success of mycorrhizal inoculation of Pinus radiate［J］. Canadian Journal of Forest Research, 17: 941—943
Garbaye J, Bowen GD, 1989. Stimulation of ectomycorrhizal infection of Pinus radiata by some microorganisms associated with the mantle of ectomycorrhizas［J］. New Phytologist, 112: 383—388
Garbaye J, Duponnois R, Wahl JL, 1990. The bacteria associated with Laccaria laccata ectomycorrhizas or sporocarps: effect on symbiosis establishment on Douglas fir［J］. Symbiosis, 9: 267—273
Garbaye J, 1991. Biological interactions in the mycorrhizosphere［J］. Experientia, 47: 370—375
Garbay J, Duponnois R, 1992. Specificity and function of mycorrhization helper bacteria (MHB) associated with the Pseudotsuga menziesiiLaccaria laccata symbiosis［J］. Symbiosis, 14: 335—344
Garbaye J, 1994. Helper bacteria: a new dimension to the mycorrhizal symbiosis［J］. New Phytologist, 128: 197—210
Gazzanelli G, Malatesta M, Pianetti A et al., 1999. Bacteria associated to fruiting bodies of the ectomycorrhizal fungus Tuber borchii Vittad［J］. Symbiosis, 26: 211—219
Glick BR, 1995. The enhancement of plant growth by free living bacteria［J］. Canadian Journal of Microbiology, 41: 109—117
Hiltner L, 1904. über neue Erfahrungen und Probleme auf dem Gebiet der Bodenbakteriologie unter besonderer Berüksichtigung der Gründüngung und Brache［J］. Arb Dtsch Landwirtsch Ges, 98: 59—78
Katznelson H, Rouatt JW, Peterson EA, 1962. The rhizosphere effect of mycorrhizal and nonmycorrhizal roots of yellow birch seedlings［J］. Canadian Journal of Botany, 40: 377—382
Linderman RG, 1988. Mycorrhizal interactions with the rhizosphere: the mycorrhizosphere effect［J］. Phytopathology, 78: 366—371
Li CY, Massicotte, HB, Moore LVH, 1992. Nitrogenfixing Bacillus sp. associated with Douglasfir tuberculate mycorrhizas［J］. Plant and Soil, 140: 35—40
Malajczuk N, McComb AJ, 1979. The microflora of unsuberized roots of Eucalyptus marginata Donn ex Sm. seedlings grown in soil suppressive and conducive to Phytophthora cinnamomi Rands. I. Rhizosphere bacteria, Actinomycetes and Fungi［J］. Australian Journal of Botany, 27: 235—254
Mamoun M, Poitou N, Olivier JM, 1986. Etude des interactions entre Tuber melanosporum Vitt. Et son environnement biotique［A］// Gianinazzi PV, Gianinazzi S eds., Mycorrhizae: Physiology and Genetics［M］. Paris: INRA, 761—765
Mello A, Miozzi L, Vizzini A et al., 2010. Bacterial and fungal communities associated with Tuber magnatum productive niches［J］. Plant Biosystems, 144: 323—332
Molina R, Massicotte H, Trappe JM, 1992. Specificity phenomena in mycorrhizal symbiosis:communityecological consequences and practical implications［A］// Allen M eds., Mycorrhizal Functioning: An Integrative PlantFungal Process［M］. UK: Chapman and Hall London, 357—423
Oswald ET, Ferchau HA, 1968. Bacterial association of coniferous mycorrhizae［J］. Plant and Soil, 28: 187—192
Pedersen EA, Reddy MS, Chakravarty P, 1999. Effect of three species of bacteria on dampingoff, root rot development, and ectomycorrhizal colonialization of lodgepole pine and white seedlings［J］. European Journal For Plant Pathology, 29: 123—134
Poole EJ, Bending GD, Whipps JM et al., 2001. Bacteria associated with Pinus sylvestrisLactarius rufus ectomycorrhizas and their effects on mycorrhiza formation in vitro［J］. New Phytologist, 151: 743—751
Probanza A, Lucas JA, Acero N et al., 1996. The influence of native rhizobacteria on European alder (Alnus glutinosa (L.) Gaertn.) growth. I. Characterization of growth promoting and growth inhibiting bacterial strains［J］. Plant and Soil, 182: 59—66
Rambelli A, 1973. The rhizosphere of mycorrhizae［A］// Marks GC, Kozlowski TT eds., Ectomycorrhizae: Their Ecology and Physiology［M］. New York: Academic, 229—343
Saitou N, Nei M, 1987. The neighborjoining method: a new method for reconstructing phylogenetic trees［J］. Molecular Biology and Evolution, 4 (4): 406—425
Sbrana C, Bagnoli G, Bedini S et al., 2000. Adhesion to hyphal matrix and antifungal activity of Pseudomonas strains isolated from Tuber borchii ascocarps［J］. Canadian Journal of Microbiology, 46: 259—268
Sbrana C, Agnolucci M, Bedini S et al., 2002. Diversity of culturable bacterial populations associated to Tuber borchii ectomycorrhizas and their activity on Tborchii mycelial growth［J］. FEMS Microbiology Letter, 211: 195—201
Schelkle M, Peterson RL, 1996. Suppression of common root pathogens by helper bacteria and ectomycorrhizal fungi in vitro［J］. Mycorrhiza, 6: 481—485
Sen R, 2000. Budgeting for the woodwide web［J］. New Phytologist, 145: 161—165
Spano SD, Jurgensen MF, Larsen MJ et al., 1982. Nitrogenfixing bacteria in Douglasfir residue decayed by Fomitopsis pinicola［J］. Plant and Soil, 68: 117—123
Tamura K, Dudley J, Nei M et al., 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 40［J］. Molecular Biology and Evolution, 24: 1596—1599
Timonen S, Jorgensen KS, Haahtela K et al., 1998. Bacterial community structure at defined locations of Pinus sylvestrisSuillus bovinus and Pinus sylvestrisPaxillus involutus mycorrhizospheres in dry pine forest humus and nursery peat［J］. Canadian Journal of Microbiology, 44: 499—513
van Tichelen KK, Colpaert JV, Vangronsveld J, 2001. Ectomycorrhizal protection of Pinus sylvestris against copper toxicity［J］. New Phytologist, 150: 203—213
Varese GC, Portinaro S, Trotta A et al., 1996. Bacteria associated with Suillus grevillei sporocarps and ectomycorrhizae and their effects on in vitro growth of the mycobiont［J］. Symbiosis, 21: 129—147
Wan SP, Liu PG, 2014. Diversity of culturable bacteria associated with ascocarps of a Chinese white truffle, Tuber panzhihuanense (Ascomycota) ［J］. Plant Diversity and Resources (植物分类与资源学报), 36: 29—36
Zacchi L, VauchanMartini A, Angelini P, 2003. Yeast distribution in a trufflefield ecosystem［J］. Annals of Microbiology, 53: 275—282