Abstract: Proteinase omega was a kind of proteinase in papaya (Carica papaya L .) laticifers . A 5′flanking sequence and partial gene sequence of proteinase omega were isolated from the genomic DNA via PCR technology . DNA sequence analysis and homology comparison indicated that it had 96% homology with the proteinase omega gene which had been submitted to GenBank . The two core promoter regions and some upstream regulatory elements in the fragment were analysed using the software of PROMOTER PREDICTION and PLANT CARE . Transcriptional start sites ( TSS) were A, T respectively . TATA-box , CAAT-box , G-box, I-box, AT rich regions and other cis- elements were found in the promoter in identical positions common to all promoter sequence regions . Compared with the data in GenBank , the results showed that a new promoter was obtained , and its GenBank accession number was AY695444 . Binary vectors were constructed through fusing 1039 bp 5′flanking region of proteinase omega gene with the GUS
gene, Transient GUS expressions were observed in papaya leaves transferred via particle bombardment . GUS activities were detected only in laticiferious cells .

Key words: Carica papaya