Plant Diversity ›› 2005, Vol. 27 ›› Issue (05): 545-551.

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Cloning and Functional Analysis of a Novel Proteinase Omega Promoter from Carica papaya

YANG Ying-Jun1 , 2 , ZHOU Peng1   

  1. 1 National Key Biotechnology Laboratory for Tropical Crops, Institute of Bioscience and Biotechnology , CATAS , Haikou , 571101 , China ; 2 Henan University of Science and Technology , Luoyang 471003 , China
  • Received:2005-03-11 Online:2005-10-01 Published:2005-10-01
  • Contact: YANG Ying-Jun

Abstract: Proteinase omega was a kind of proteinase in papaya (Carica papaya L .) laticifers . A 5′flanking sequence and partial gene sequence of proteinase omega were isolated from the genomic DNA via PCR technology . DNA sequence analysis and homology comparison indicated that it had 96% homology with the proteinase omega gene which had been submitted to GenBank . The two core promoter regions and some upstream regulatory elements in the fragment were analysed using the software of PROMOTER PREDICTION and PLANT CARE . Transcriptional start sites ( TSS) were A, T respectively . TATA-box , CAAT-box , G-box, I-box, AT rich regions and other cis- elements were found in the promoter in identical positions common to all promoter sequence regions . Compared with the data in GenBank , the results showed that a new promoter was obtained , and its GenBank accession number was AY695444 . Binary vectors were constructed through fusing 1039 bp 5′flanking region of proteinase omega gene with the GUS
gene, Transient GUS expressions were observed in papaya leaves transferred via particle bombardment . GUS activities were detected only in laticiferious cells .

Key words: Carica papaya