Plant Diversity ›› 2005, Vol. 27 ›› Issue (05): 552-556.

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Induction of Polyploid of Dendrobium devonianum

LI Han1 , 2 , ZHENG Si-Xiang1 , LONG Chun-Lin2   

  1. 1 College of Horticulture , Yunnan Agriculture University , Kunming 650201 , China ; 2 Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204 , China
  • Received:2005-01-17 Online:2005-10-01 Published:2005-10-01
  • Contact: LONG Chun-Lin

Abstract: The polyploid breeding technique was used to induce polyploid of Dendrobium devonianum, an orchid occurring in the tropics of Yunnan with ornamental and medicinal values . The materials for polyploid breeding were in vitro seedlings of diploids . The polyploid plants could be induced in a short time with colchicines as mutagen . It resulted in the best inductive effect to use 0 . 03% colchicines to deal with the in vitro seedlings of diploids for 24 hours . The polyploid plants were different from those of diploids in morphology , stoma diameter , chromosome numbers, and DNA fingerprints . The in vitro seedlings of polyploid plant showed advantageous characteristics such as thicker stems and leaves . It is a successful case to apply polyploid breeding technique into the tropical orchid breeding in China .

Key words: Dendrobium devonianum