Plant Diversity ›› 2005, Vol. 27 ›› Issue (05): 557-564.

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Metabolic Profiling Analysis of Taxanes in Taxus chinensis Cell Cultures Elicited by Methyl Jasmonate

ZHAO Chun-Fang, YU Long-Jiang, LIU Zhi , LI Mao-Teng ,
ZHOU Peng-Peng , LI Li-Qin   

  1. School of Life Science and Technology , Huazhong University of Science and Technology
  • Received:2005-01-07 Online:2005-10-01 Published:2005-10-01
  • Contact: YU Long-Jiang

Abstract: Taxus chinensis cell cultures were extracted with methanol and then prepared by solid-phase extract prior to HPLC or LC-MS analysis . About 13 taxane compounds were identified or characterized by comparisons with LC-MS and standard referents . The profiling analysis of the taxanes by methyl jasmonate (MJ ) elicitation indicated that the relative chromatographic peak areas of all these identified compounds were increased but the increasing rates differed . Taxchinin M and its structural analogue had the highest increment . The increasing rates of taxol, B -Ⅲ and B -Ⅵ were more than that of taxuyunnanine C and its derivatives, which were oxygenated at C-14 . The results suggested that biosynthsis of taxol and its precursors were more sensitive than oxygenated taxanes at C-14 in response to methyl jasmonate elicitations, while MJ may also elicit the formation of non-effective acetylated taxanes which bear acetate groups at C1 ,C2 , C7 , C9 and C13 . The study may be interesting in metabolic profiling analysis for elucidating taxane biosynthesis .

Key words: Taxanes