Plant Diversity ›› 2005, Vol. 27 ›› Issue (06): 669-675.

• Articles • Previous Articles     Next Articles

Expression of VP1 of the Food-and-Mouth Disease Virus Outer Capsid Protein in Transgenic Stylosanthes spp.

WANG Dong-Mei , SHEN Wen-Tao, ZHOU Peng   

  1. State Key Laboratory of Tropical Crop Biotechnology, Institute of Tropical Biology Sciences, China Academy of Tropical Agricultural Science , Haikou 571101 , China
  • Received:2005-03-30 Online:2005-10-25 Published:2005-10-25
  • Contact: ZHOU Peng

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Abstract: Tender leaves of Stylosanthes guianensis cv . Reyan Ⅱ were transformed with Agrobacterium tumefaciens LBA4404 carrying the gene VP1 coding food-and-mouth disease virus outer capsid protein . After 3 times kanamycin-resistant selection , the regenerated plantlets were analyzed further by PCR, PCRSouthern blot and Southern blot, the results confirmed that the VP1 gene were integrated in the genome . The results of RT-PCR and Northern blot of transgenic plants showed that VP1 gene was transcribed in transgenic plants .

Key words: FMDV

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