Plant Diversity ›› 2006, Vol. 28 ›› Issue (06): 593-598.

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The Ployploid Induction and Identification of Platycodon grandiflorus (Campanulaceae) in China

WANG Xiao-Hua; XIONG Li; QU Yun-Hui; YANG Jing; GU Zhi-Jian   

  1. Yunnan Agricultural University
  • Received:2006-02-06 Online:2006-12-25 Published:2006-12-25
  • Contact: WANG Xiao-Hua

Abstract: In in vitro culture , Platycodon grandiflorus was treated with colchicine at different concentration and time duration. It showed that the induction rate could reach 50% with colchicine at 0.1% for 40 h. Compared with the normal
diploid plants, the polyploid plants had thicker leaves and larger leaves, darker leaf color , stronger stems with longer length per stem stalk, larger stomata and fewer stomata per unit area. Cytological observation showed the chromosome number of variant plants is 2n=4x=36, while that of normal diploid plant is 2n=2x=18, x=9. Therefore, the variant plants (2n= 4x=36) belong to tetraploid plants. Karyotype formula of the former is 2n=2x=14m+ 20sm+ 2st, and that of the latter is 2n=4x=7m+10sm+1st. Both of the karyotype belong to Stebbins’s 2B type. It was also found that a few induced plants were aneuploid .

Key words: Platycodon grandiflorus