Plant Diversity ›› 2006, Vol. 28 ›› Issue (2): 194-198.

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The Effect of Polyamines on the Floral Bud Construction of in vitro Cotyledons in Cucumis sativus ( Cucurbitaceae)

 HUANG  Zuo-Chi-1、2,   Tang-Zheng-Yi-1,   Xie-Yin-Feng-3,   Duan-Hui-Guo-1,   Qing-Dong-Hong-1   

  1. 1 Department of Chemistry and Lif e Science , Neijiang Normal College , Neijiang 641112, China; 2 Flower Research Institute of
    Neijiang Normal College , Neijiang 641112, China; 3 College of Forest Resources and Environment ,
    Nanjing Forestry University , Nanjing 210037, China
  • Online:2006-04-25 Published:2014-01-17


The effects of exogenous polyamines on the levels of polyamines in the in vitro cotyledons and on the floral bud
construction were examined in Cucumis sativus . Application of 5 @ 10- 2 mmol#L- 1 spermidine ( Spd) and spermine
( Spm) inMS culture medium remarkably increases the levels of Spd and the ratio of SpdPputrescine ( Put) in cotyledons
and results in the rate of floral buds higher than that of control; however, application of 5@ 10- 2 mmol#L- 1 Put or 5 mmol#L- 1
CHAS leads to opposite results, suggesting that the levels of Spd and ratio of SpdPPut in cotyledons are positive correlated
with the rate of floral buds. Application of 5mmol#L- 1 D-Arg in medium results in that the ratio of SpdPPut is higher than
that treated with Spd and that rate of floral buds in cotyledons is lower than that of control, indicating that the levels of Put
and Spd have effects on the construction of floral buds. Comparing with control, application of CHAS increases the levels
of Spm in cotyledons 10 folds and decreases the rate of floral buds to zero. There is no significant difference in the levels
of endogenous Spm between other treatments and control, except the rate of floral buds, meaning there is no correlation
between the levels of Spm in cotyledons and the construction of floral buds in Cucumis sativus .

Key words: Cucumis sativus , Polyamines, Cotyledon, in vitr o, Floral bud construction

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