Plant Diversity ›› 2006, Vol. 28 ›› Issue (2): 203-207.

• Articles • Previous Articles     Next Articles

Improving of in vitro Plant Regeneration Frequency of Rosa canina ( Rosaceae) Inermis

 YU  Xiao-Li-1,   Ma-Jian-Min-2,   Li-Yu-Ying-1,   Wang-Shi-Ru-3   

  1. 1 Department of Biol ogy, Nanyang Normal University, Nanyang 473061, China; 2 Coll ege of Lif e Sci ence of Henan Normal University,
    Xinxiang 453002, China; 3 Department of Horticulture , Nanyang Agricultural School , Nanyang 473000, China
  • Online:2006-04-25 Published:2014-01-17


An efficient regeneration system of Rosa canina Inermis was developed in this study. The results showed that
the regeneration frequency of adventitious buds was 87% and the multiplication ratio was 310 when supplemented with 210
mgPL 6-BA and 013 mgPL 2, 4-D onMurashige and Skoog (MS) medium, while the regeneration ratio was 93% and the
multiplication ratio was 510 when the MS medium contained 115 mgPL CPPU and 012 mgPL 2, 4-D. Moreover , the result
also indicated that CPPU was more effective in the induction of adventitious buds. The optimal medium for the multiplication
of shoots wasMS+ 40gPL sucrose+ 610 gPL agar+ 315 mgPL AgNO3 + 11 5mgPL CPPU+ 011 mgPL 2, 4-D+ 01 05mgP
L GA3 , in which the multiplication ratio was 515 and the adventitious bud induction frequency was 89%. The best med-i
um for rooting and growing was 1P4MS+ 20 gPL sucrose+ 31 5gPL agar+ 01 3% activated carbon+ 011 mgPL IBA+ 011mgP
LNAA. The rooting frequency was 91%.

Key words: Rosa canina, Plant regeneration, Tissue culture

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