Plant Diversity ›› 2007, Vol. 29 ›› Issue (02): 243-246.

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IGS1-RFLP Comparative Analysis of Tricholoma matsutake (Tricholomataceae) from Yunnan and Japan

SHA Tao1 , QIANG Yong2 , ZHANG Han-Bo1 , 2 , DING Hua-Sun2 ,
CHENG Li-Zhong2 , ZHAO Zhi-Wei1   

  1. 1 Laboratory for Conservation and Utilization of Bio-resources, Yunnan University , Kunming 650091 , China ;
    2 Department of Biology, Yunnan University , Kunming 650091 , China
  • Received:2006-06-13 Online:2007-04-25 Published:2007-04-25
  • Contact: SHA Tao

Abstract: IGS1 genes were obtained by PCR amplification from genome DNA of Tricholoma matsutake fruit bodies collected from 16 counties in Yunnan . These gene fragments were digested by restriction endonuclease Cfr13Ⅰ . Two distinct IGS1 rDNA types were identified , namely A and C . Among the 127 fruit bodies investigated , 126 belong to type A while one from Jianchuan (T89) belongs to type C . Our results also showed that there are no significant heterogeneity in the IGS1 genes of these fruit bodies , except double CTTTs found in representative IGS1 gene of type A . The primary IGS1 RFLP patterns of Tricholoma matsutake from Yunnan are very similar to those from Japanese , implying that Tricholoma matsutake of these two areas are likely to be homologous .

Key words: Tricholoma matsutake