Plant Diversity ›› 2007, Vol. 29 ›› Issue (02): 251-255.

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Extraction, Separation and Measuration of the Thermal Hysteresis Activity of Antifreeze Proteins from Ammopiptanthus nanus (Leguminosae)

YU Shan-Shan , YIN Lin-Ke* * , MU Shu-Yong, LU Chun-Fang , ZHAO Feng-Xia   

  1. Xinjiang Institute of Ecology and Geography , Chinese Academy of Sciences, Urumqi 830011 , China
  • Received:2006-06-20 Online:2007-04-25 Published:2007-04-25
  • Contact: YIN Lin-Ke

Abstract: Antifreeze proteins (AFPs) , because of their ability of adsorbing onto the surface of ice crystals and modifying their growth , have been found in many different organisms that are exposed to chilling and freezing temperatures . It is very important to increase the resisting and coercing ability at low temperature . DE-52 ion- exchange cellulose was used to extract and separate the antifreeze proteins from Ammopiptanthus nanus leaves. Differential scanning calorimetry (DSC) was used to measure the thermal hysteresis activity (THA) . The results reveal that when the concentration of the protein is 20mg/ml , the THA of the AFPs is 0 . 46℃ . Its molecular weight is about 119. 24 kDa by sodium dodecyl sulfate polyacrylam ide gel electrophoresis (SDS-PAGE) .

Key words: Ammopiptanthus nanus